PUBLICATION
LC-MS analysis of ginsenosides in different parts of Panax quinquefolius and their potential for coronary disease improvement
- Authors
- Zhang, X., Kong, C., Wang, X., Hou, H., Yu, H., Wang, L., Li, P., Li, X., Zhang, Y., Han, L., Liu, K.
- ID
- ZDB-PUB-230321-46
- Date
- 2023
- Source
- Planta Medica 89(7): 764-772 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromatography, High Pressure Liquid
- Chromatography, Liquid
- Coronary Artery Disease*
- Endothelial Cells
- Ginsenosides*/pharmacology
- Panax*
- Plant Extracts/pharmacology
- Tandem Mass Spectrometry
- Vascular Endothelial Growth Factor A
- Zebrafish
- PubMed
- 36940929 Full text @ Planta Med.
Citation
Zhang, X., Kong, C., Wang, X., Hou, H., Yu, H., Wang, L., Li, P., Li, X., Zhang, Y., Han, L., Liu, K. (2023) LC-MS analysis of ginsenosides in different parts of Panax quinquefolius and their potential for coronary disease improvement. Planta Medica. 89(7):764-772.
Abstract
Seven main ginsenosides, including ginsenoside Re, ginsenoside Rb1, pseudoginsenoside F11, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd and ginsenoside F2, were identified by LC-QTOF MS/MS from root, leaf and flower extracts of Panax quinquefolius. These extracts promoted intersegmental vessel growth in a zebrafish model, indicating their potential cardiovascular health benefits. Network pharmacology analysis was then conducted to reveal the potential mechanisms of ginsenoside activity in the treatment of coronary artery disease. GO and KEGG enrichment analyses elucidated that G protein-coupled receptors played a critical role in VEGF-mediated signal transduction and that the molecular pathways associated with ginsenoside activity are involved in neuroactive ligand-receptor interaction, cholesterol metabolism and the cGMP-PKG signaling pathway, etc. Moreover, VEGF, FGF2, and STAT3 were confirmed as the major targets inducing proliferation of endothelial cells and driving the pro-angiogenic process. Overall, ginsenosides could be potent nutraceutical agents that act to reduce the risks of cardiovascular disease. Our findings will provide a basis to utilize the whole P. quinquefolius plant in drugs and functional foods.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping