PUBLICATION
Accumulation, metabolites formation and elimination behavior of rac-glufosinate-ammonium and glufosinate-P in zebrafish (Danio rerio)
- Authors
- Wang, F., Lin, Q., Shi, X., Li, Y., Deng, P., Zhang, Y., Hu, D.
- ID
- ZDB-PUB-221018-12
- Date
- 2022
- Source
- Food chemistry: X 15: 100383 (Journal)
- Registered Authors
- Keywords
- Bioaccumulation, Degradation, Glufosinate-P, Metabolites, Rac-glufosinate-ammonium, Zebrafish
- MeSH Terms
- none
- PubMed
- 36211745 Full text @ Food Chem X
Citation
Wang, F., Lin, Q., Shi, X., Li, Y., Deng, P., Zhang, Y., Hu, D. (2022) Accumulation, metabolites formation and elimination behavior of rac-glufosinate-ammonium and glufosinate-P in zebrafish (Danio rerio). Food chemistry: X. 15:100383.
Abstract
An efficient trace detection method for the determination of residues of the glufosinate enantiomers and metabolites in zebrafish by HPLC-Q-Exactive Orbitrap Mass Spectrometry was developed. After the purification of dichloromethane and Oasis PRiME HLB SPE column, the recovery ranges from 77% to 104%, with RSD < 10.03%. The limits of quantitation in zebrafish were 0.006-0.02 mg/kg. The results revealed zebrafish absorbed glufosinate slowly, reaching a steady state in 10-14 days, and the bioaccumulation factor (BCF) of D/L-glufosinate-ammonium was less than 0.3. L-glufosinate-ammonium accumulated preferentially in zebrafish. The residue of the metabolite N-acetyl glutamate (NAG) was smaller than that of 3-methyl phosphonic acid (MPP). D/L-glufosinate-ammonium had an elimination half-life of less than 2.3 days during the elimination phase. The bioaccumulation and elimination behavior of glufosinate-ammonium in zebrafish aquatic system was shown in this work, which offered scientific data for assessing the food safety of rac-glufosinate-ammonium and glufosinate-P (pure L-glufosinate-ammonium) in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping