PUBLICATION
Clitorienolactones and Isoflavonoids of Clitorea ternatea Roots Alleviate Stress-Like Symptoms in a Reserpine-Induced Zebrafish Model
- Authors
- Ngadni, M.A., Akhtar, M.T., Ismail, I.S., Norazhar, A.I., Lee, S.Y., Maulidiani, M., Shaari, K.
- ID
- ZDB-PUB-210728-10
- Date
- 2021
- Source
- Molecules 26(14): (Journal)
- Registered Authors
- Keywords
- Clitorea ternatea, LCMS/MS, clitorienolactones, flavonoids, metabolite profile, novel tank test, reserpine-induced stress, zebrafish model
- MeSH Terms
-
- Animals
- Behavior, Animal
- Clitoria/chemistry*
- Depression/chemically induced
- Depression/drug therapy*
- Flavonoids/pharmacology*
- Isoflavones/pharmacology
- Lactones/pharmacology*
- Plant Extracts/pharmacology*
- Plant Roots/chemistry*
- Reserpine/toxicity
- Stress, Physiological/drug effects*
- Zebrafish
- PubMed
- 34299411 Full text @ Molecules
Citation
Ngadni, M.A., Akhtar, M.T., Ismail, I.S., Norazhar, A.I., Lee, S.Y., Maulidiani, M., Shaari, K. (2021) Clitorienolactones and Isoflavonoids of Clitorea ternatea Roots Alleviate Stress-Like Symptoms in a Reserpine-Induced Zebrafish Model. Molecules. 26(14):.
Abstract
Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated using ultrahigh-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD-MS/MS), while their effect on the stress-like behavior of zebrafish, pharmacologically induced with reserpine, was investigated. A total of 32 compounds were putatively identified, among which, a series of norneolignans, clitorienolactones, and various flavonoids (flavone, flavonol, isoflavone, and isoflavanone) was found to comprise the major constituents, particularly in the EA and 50% MeOH fractions. The clitorienolactones, presently unique to the species, were present in both the free and glycosylated forms in the roots. Both the EA and 50% MeOH fractions displayed moderate effects on the stress-induced zebrafish model, significantly decreasing freezing duration and elevating the total distance travelled and average velocity, 72 h post-treatment. The results of the present study provide further evidence that the basis for the use of C. ternatea roots in traditional medicine to alleviate brain-related conditions, such as stress and depression, is attributable to the presence of clitorienolactones and the isoflavonoidal constituents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping