PUBLICATION
CTPS forms the cytoophidium in zebrafish
- Authors
- Chang, C.C., Keppeke, G.D., Antos, C.L., Peng, M., Coelho Andrade, L.E., Sung, L.Y., Liu, J.L.
- ID
- ZDB-PUB-210616-34
- Date
- 2021
- Source
- Experimental cell research 405(2): 112684 (Journal)
- Registered Authors
- Keywords
- CTPS, cytoophidium, zebrafish
- MeSH Terms
-
- Animals
- Carbon-Nitrogen Ligases/metabolism*
- Cell Line
- Cytidine Triphosphate/metabolism*
- Eukaryota/cytology*
- Nitric Oxide Synthase/metabolism
- Prokaryotic Cells/cytology*
- Zebrafish
- PubMed
- 34129847 Full text @ Exp. Cell Res.
Citation
Chang, C.C., Keppeke, G.D., Antos, C.L., Peng, M., Coelho Andrade, L.E., Sung, L.Y., Liu, J.L. (2021) CTPS forms the cytoophidium in zebrafish. Experimental cell research. 405(2):112684.
Abstract
Cytidine triphosphate synthase (CTPS) catalyzes the rate-limiting step of de novo CTP biosynthesis. An intracellular structure of CTPS, the cytoophidium, has been found in many organisms including prokaryotes and eukaryotes. Formation of the cytoophidium has been suggested to regulate the activity and stability of CTPS and may participate in certain physiological events. Herein, we demonstrate that both CTPS1a and CTPS1b in zebrafish are able to form the cytoophidium in cultured cells. A point mutation, H355A, abrogates cytoophidium assembly of zebrafish CTPS1a and CTPS1b. In addition, we show the presence of CTPS cytoophidia in multiple tissues of larval and adult fish under normal conditions, while treatment with a CTPS inhibitor 6-diazo-5-oxo-L-norleucine (DON) can induce more cytoophidia in some tissues. Our findings reveal that forming the CTPS cytoophidium is a natural phenomenon of zebrafish and provide valuable information for future research on the physiological importance of this intracellular structure in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping