PUBLICATION
Melatonin administration attenuates acute stress by inducing sleep state in zebrafish (Danio rerio)
- Authors
- Lunkes, L.C., Paiva, I.M., Egger, R.C., Braga, W.F., Alvarez-Leite, J.I., da Cunha Barreto-Vianna, A.R., Murgas, L.D.S.
- ID
- ZDB-PUB-210411-7
- Date
- 2021
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 246: 109044 (Journal)
- Registered Authors
- Keywords
- Anxiety, Behavior, Fish, Hormone, Melatonin, Oxidative stress
- MeSH Terms
-
- Animals
- Antioxidants/pharmacology*
- Hydrocortisone/metabolism
- Melatonin/pharmacology*
- Oxidative Stress/drug effects*
- Sleep/drug effects*
- Zebrafish
- PubMed
- 33838315 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Lunkes, L.C., Paiva, I.M., Egger, R.C., Braga, W.F., Alvarez-Leite, J.I., da Cunha Barreto-Vianna, A.R., Murgas, L.D.S. (2021) Melatonin administration attenuates acute stress by inducing sleep state in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 246:109044.
Abstract
Melatonin plays a fundamental homeostatic role in basic biological functions, and an anti-stress role has been also proposed for this hormone. This study aimed to evaluate hormonal, enzymatic and behavioral parameters of zebrafish that received administration of melatonin and were submitted to acute stress. A total of 120 wild-type zebrafish were divided into five groups: naïve control (N), negative control group (Stress/C), positive control treated with diazepam (Stress/Diaz), treatment with melatonin at dose 1 (Stress/Melt. 1) and treatment with melatonin at dose 2 (Stress/Melt. 2). The exposure to treatments (diazepam or melatonin) was performed prior to the acute stress protocol, based on a chase by a fishing net during 5 min followed by exposure to the air for 1 min. The body cortisol levels were assessed, as well as oxidative stress (thiobarbituric acid reactive substances, reactive species of oxygen and antioxidant activity), and fish behavior (open field test). Melatonin was able to modulate acute stress effects on zebrafish by inhibiting cortisol increasing levels, reducing locomotor parameters, inducing a sleep state, reducing lipid peroxidation and stimulating antioxidant enzymatic activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping