PUBLICATION
Behavioral Effects of Developmental Exposure to JWH-018 in Wild-Type and Disrupted in Schizophrenia 1 (disc1) Mutant Zebrafish
- Authors
- García-González, J., de Quadros, B., Havelange, W., Brock, A.J., Brennan, C.H.
- ID
- ZDB-PUB-210307-8
- Date
- 2021
- Source
- Biomolecules 11(2): (Journal)
- Registered Authors
- Keywords
- JWH-018, anxiety, cannabinoids, disc1, forced light/dark assay, zebrafish
- MeSH Terms
-
- Anxiety/etiology
- Maternal Exposure
- Nerve Tissue Proteins/genetics*
- Acoustics
- Naphthalenes/adverse effects*
- Cannabinoids/adverse effects
- Behavior, Animal/drug effects
- Zebrafish/embryology*
- Alleles
- Animals
- Nicotine
- Gene Expression Regulation, Developmental*
- Dronabinol/adverse effects*
- Zebrafish Proteins/genetics*
- Indoles/adverse effects*
- Mutation
- Movement/drug effects
- Female
- Models, Genetic
- PubMed
- 33669793 Full text @ Biomolecules
Citation
García-González, J., de Quadros, B., Havelange, W., Brock, A.J., Brennan, C.H. (2021) Behavioral Effects of Developmental Exposure to JWH-018 in Wild-Type and Disrupted in Schizophrenia 1 (disc1) Mutant Zebrafish. Biomolecules. 11(2):.
Abstract
Synthetic cannabinoids can cause acute adverse psychological effects, but the potential impact when exposure happens before birth is unknown. Use of synthetic cannabinoids during pregnancy may affect fetal brain development, and such effects could be moderated by the genetic makeup of an individual. Disrupted in schizophrenia 1 (DISC1) is a gene with important roles in neurodevelopment that has been associated with psychiatric disorders in pedigree analyses. Using zebrafish as a model, we investigated (1) the behavioral impact of developmental exposure to 3 μM 1-pentyl-3-(1-naphthoyl)-indole (JWH-018; a common psychoactive synthetic cannabinoid) and (2) whether disc1 moderates the effects of JWH-018. As altered anxiety responses are seen in several psychiatric disorders, we focused on zebrafish anxiety-like behavior. Zebrafish embryos were exposed to JWH-018 from one to six days post-fertilization. Anxiety-like behavior was assessed using forced light/dark and acoustic startle assays in larvae and novel tank diving in adults. Compared to controls, both acutely and developmentally exposed zebrafish larvae had impaired locomotion during the forced light/dark test, but anxiety levels and response to startle stimuli were unaltered. Adult zebrafish developmentally exposed to JWH-018 spent less time on the bottom of the tank, suggesting decreased anxiety. Loss-of-function in disc1 increased anxiety-like behavior in the tank diving assay but did not alter sensitivity to JWH-018. Results suggest developmental exposure to JWH-018 has a long-term behavioral impact in zebrafish, which is not moderated by disc1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping