PUBLICATION
Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
- Authors
- Bieczynski, F., Burkhardt-Medicke, K., Marcelo Luquet, C., Scholz, S., Luckenbach, T.
- ID
- ZDB-PUB-201208-26
- Date
- 2020
- Source
- FEBS letters 595(6): 828-843 (Journal)
- Registered Authors
- Keywords
- ABC efflux transporter, ATPase activity, chemosensitizer, dye accumulation assay, recombinant protein, zebrafish Abcb4, zebrafish embryo
- MeSH Terms
-
- ATP-Binding Cassette Transporters/chemistry*
- ATP-Binding Cassette Transporters/genetics
- ATP-Binding Cassette Transporters/metabolism*
- Animals
- Biological Transport, Active
- Recombinant Proteins/chemistry
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Rhodamines/chemistry
- Rhodamines/pharmacokinetics*
- Rhodamines/pharmacology
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 33274443 Full text @ FEBS Lett.
Citation
Bieczynski, F., Burkhardt-Medicke, K., Marcelo Luquet, C., Scholz, S., Luckenbach, T. (2020) Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity. FEBS letters. 595(6):828-843.
Abstract
ATP binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy-based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium-throughput fashion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping