PUBLICATION
Spastin mutations impair coordination between lipid droplet dispersion and reticulum
- Authors
- Arribat, Y., Grepper, D., Lagarrigue, S., Qi, T., Cohen, S., Amati, F.
- ID
- ZDB-PUB-200422-174
- Date
- 2020
- Source
- PLoS Genetics 16: e1008665 (Journal)
- Registered Authors
- Amati, Francesca, Arribat, Yoan, Grepper, Dogan, Lagarrigue, Silviane
- Keywords
- none
- MeSH Terms
-
- Animals
- Humans
- Zebrafish
- Cells, Cultured
- Microtubules/metabolism
- GTP-Binding Protein gamma Subunits/metabolism
- Membrane Transport Proteins/metabolism
- Spastin/genetics
- Spastin/metabolism*
- Endoplasmic Reticulum/metabolism*
- Lipid Droplets/metabolism*
- HeLa Cells
- Protein Binding
- PubMed
- 32315314 Full text @ PLoS Genet.
Citation
Arribat, Y., Grepper, D., Lagarrigue, S., Qi, T., Cohen, S., Amati, F. (2020) Spastin mutations impair coordination between lipid droplet dispersion and reticulum. PLoS Genetics. 16:e1008665.
Abstract
Lipid droplets (LD) are affected in multiple human disorders. These highly dynamic organelles are involved in many cellular roles. While their intracellular dispersion is crucial to ensure their function and other organelles-contact, underlying mechanisms are still unclear. Here we show that Spastin, one of the major proteins involved in Hereditary Spastic Paraplegia (HSP), controls LD dispersion. Spastin depletion in zebrafish affects metabolic properties and organelle dynamics. These functions are ensured by a conserved complex set of splice variants. M1 isoforms determine LD dispersion in the cell by orchestrating endoplasmic reticulum (ER) shape along microtubules (MTs). To further impact LD fate, Spastin modulates transcripts levels and subcellular location of other HSP key players, notably Seipin and REEP1. In pathological conditions, mutations in human Spastin M1 disrupt this mechanism and impacts LD network. Spastin depletion influences not only other key proteins but also modulates specific neutral lipids and phospholipids, revealing an impact on membrane and organelle components. Altogether our results show that Spastin and its partners converge in a common machinery that coordinates LD dispersion and ER shape along MTs. Any alteration of this system results in HSP clinical features and impacts lipids profile, thus opening new avenues for novel biomarkers of HSP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping