PUBLICATION
PKC Induces Release of a Functional Ectodomain of the Guidance Cue Semaphorin6A
- Authors
- St Clair, R.M., Dumas, C.M., Williams, K.S., Goldstein, M.T., Stant, E.A., Ebert, A.M., Ballif, B.A.
- ID
- ZDB-PUB-190807-3
- Date
- 2019
- Source
- FEBS letters 593(21): 3015-3028 (Journal)
- Registered Authors
- Keywords
- PKC, Eye Development, Guidance Cue, Semaphorin, Semaphorin6A, Zebrafish
- MeSH Terms
-
- Protein Domains
- Humans
- Serine/chemistry
- Mass Spectrometry
- Phosphorylation
- HEK293 Cells
- Animals
- Mice
- Protein Kinase C/metabolism*
- Semaphorins/chemistry*
- Semaphorins/genetics
- Semaphorins/metabolism*
- Gene Expression Regulation
- Frontal Lobe/cytology
- Frontal Lobe/metabolism*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 31378926 Full text @ FEBS Lett.
Citation
St Clair, R.M., Dumas, C.M., Williams, K.S., Goldstein, M.T., Stant, E.A., Ebert, A.M., Ballif, B.A. (2019) PKC Induces Release of a Functional Ectodomain of the Guidance Cue Semaphorin6A. FEBS letters. 593(21):3015-3028.
Abstract
Semaphorins (Semas) are a family of secreted and transmembrane proteins that play critical roles in development. Interestingly, several vertebrate transmembrane Sema classes are capable of producing functional soluble ectodomains. However, little is known of soluble Sema6 ectodomains in the nervous system. Herein, we show that the soluble Sema6A ectodomain, sSema6A, exhibits natural and PKC-induced release. We show that PKC mediates Sema6A phosphorylation at specific sites and while this phosphorylation is not the primary mechanism regulating sSema6A production, we found that the intracellular domain confers resistance to ectodomain release. Finally, sSema6A is functional as it promotes the cohesion of zebrafish early eye field explants. This suggests that in addition to its canonical contact-mediated functions, Sema6A may have regulated, long-range, forward-signaling capacity. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping