PUBLICATION
Folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks: fluorescence and magnetic resonance dual-modality imaging and photodynamic therapy in hepatocellular carcinoma
- Authors
- Chen, Y., Liu, W., Shang, Y., Cao, P., Cui, J., Li, Z., Yin, X., Li, Y.
- ID
- ZDB-PUB-181228-5
- Date
- 2018
- Source
- International Journal of Nanomedicine 14: 57-74 (Journal)
- Registered Authors
- Keywords
- dual-modality imaging, folic acid, hepatocellular carcinoma, nanoscale gadolinium-porphyrin metal-organic frameworks, photodynamic therapy
- MeSH Terms
-
- Animals
- Carcinoma, Hepatocellular/drug therapy*
- Carcinoma, Hepatocellular/pathology
- Cell Death
- Cell Survival/drug effects
- Embryo, Nonmammalian/drug effects
- Fluorescence
- Folic Acid/chemistry*
- Folic Acid/therapeutic use*
- Gadolinium/chemistry*
- Hep G2 Cells
- Humans
- Liver Neoplasms/drug therapy*
- Liver Neoplasms/pathology
- Magnetic Resonance Imaging*
- Metal-Organic Frameworks/chemical synthesis
- Metal-Organic Frameworks/chemistry*
- Nanoparticles/chemistry
- Nanoparticles/ultrastructure
- Photochemotherapy*
- Time-Lapse Imaging
- Zebrafish/embryology
- PubMed
- 30587985 Full text @ Int. J. Nanomedicine
Citation
Chen, Y., Liu, W., Shang, Y., Cao, P., Cui, J., Li, Z., Yin, X., Li, Y. (2018) Folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks: fluorescence and magnetic resonance dual-modality imaging and photodynamic therapy in hepatocellular carcinoma. International Journal of Nanomedicine. 14:57-74.
Abstract
Background Hepatocellular carcinoma (HCC) is the most common primary liver cancer and severely threatens human health. Since the prognosis of advanced HCC remains poor, there is an urgent need to develop new therapeutic approaches. Porphyrin metal-organic frameworks are a class of porous organic-inorganic hybrid functional materials with good biocompatibility.
Methods Gadolinium-porphyrin metal-organic frameworks were used as a skeleton for folic acid (FA) conjugation to synthesize a novel type of nanoparticle, denoted as folic acid-nanoscale gadolinium-porphyrin metal-organic frameworks (FA-NPMOFs). The FA-NPMOFs were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric-differential thermal analysis. The biotoxicity and imaging capability of the FA-NPMOFs were determined using HepG2 cells and embryonic and larval zebrafish. The delivery and photodynamic therapeutic effect of FA-NPMOFs were explored in transgenic zebrafish with doxycycline-induced HCC.
Results FA-NPMOFs were spherical in structure with good dispersion and water solubility. They showed low biotoxicity, emitted bright red fluorescence, and exhibited an excellent magnetic resonance imaging capability, both in vitro and in vivo. Meanwhile, the FA-NPMOFs exhibited a strong affinity for folate receptor (FR)-expressing cells and were delivered to the tumor site in a targeted manner. Moreover, HCC tumor cells were eliminated following laser irradiation.
Conclusion FA-NPMOFs can be used for dual-modality imaging and photodynamic therapy in HCC and show promise for use as a carrier in new therapies for HCC and other FR-positive tumors.
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