PUBLICATION
Bactericidal synergism between antibiotics and phage endolysin Cpl-711 to kill multidrug-resistant pneumococcus
- Authors
- Letrado, P., Corsini, B., Díez-Martínez, R., Bustamante, N., Yuste, J.E., García, P.
- ID
- ZDB-PUB-180922-7
- Date
- 2018
- Source
- Future Microbiology 13: 1215-1223 (Journal)
- Registered Authors
- Keywords
- Streptococcus pneumoniae, antibiotic multidrug-resistant, endolysin, mouse model, synergy, zebrafish model
- MeSH Terms
-
- Animals
- Zebrafish
- Drug Resistance, Multiple, Bacterial*
- Sepsis/drug therapy
- Sepsis/microbiology*
- Cefotaxime/pharmacology
- Cefotaxime/therapeutic use
- Pneumococcal Infections/drug therapy
- Pneumococcal Infections/microbiology*
- Streptococcus pneumoniae/drug effects*
- Streptococcus Phages/enzymology
- Drug Synergism
- Muramidase/pharmacology*
- Muramidase/therapeutic use
- Disease Models, Animal
- Anti-Bacterial Agents/pharmacology*
- Anti-Bacterial Agents/therapeutic use
- Mice, Inbred BALB C
- Mice
- Female
- Recombinant Fusion Proteins/pharmacology*
- Recombinant Fusion Proteins/therapeutic use
- PubMed
- 30238774 Full text @ Future Microbiol.
Citation
Letrado, P., Corsini, B., Díez-Martínez, R., Bustamante, N., Yuste, J.E., García, P. (2018) Bactericidal synergism between antibiotics and phage endolysin Cpl-711 to kill multidrug-resistant pneumococcus. Future Microbiology. 13:1215-1223.
Abstract
Aim To test the synergistic effect of Cpl-711 endolysin and antibiotics for antipneumococcal activity.
Materials & methods A combination of Cpl-711 and different antibiotics (amoxicillin, cefotaxime, levofloxacin and vancomycin) was tested in a checkerboard assay against several multidrug-resistant Streptococcus pneumoniae strains. Mouse and zebrafish models of pneumococcal sepsis were used to confirm the in vitro data.
Results The activity of Cpl-711 combined with amoxicillin or cefotaxime was synergistic in the bactericidal effect against a serotype 23F multiresistant clinical isolate of S. pneumoniae. Synergy between Cpl-711 and cefotaxime was validated using both mouse and zebrafish models.
Conclusion Combination of Cpl-711 and cefotaxime may help in the treatment of diseases caused by multiresistant pneumococcal strains.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping