PUBLICATION

Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry

Authors
Juan, T., Géminard, C., Coutelis, J.B., Cerezo, D., Polès, S., Noselli, S., Fürthauer, M.
ID
ZDB-PUB-180518-3
Date
2018
Source
Nature communications   9: 1942 (Journal)
Registered Authors
Fürthauer, Maximilian, Juan, Thomas, Polès, Sophie
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/genetics*
  • Cell Polarity/genetics
  • Cilia/genetics
  • Cilia/metabolism
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Left-Right Determination Factors/genetics
  • Left-Right Determination Factors/metabolism
  • Mutation
  • Myosins/genetics*
  • Myosins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
29769531 Full text @ Nat. Commun.
Abstract
The establishment of left-right (LR) asymmetry is fundamental to animal development, but the identification of a unifying mechanism establishing laterality across different phyla has remained elusive. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, LR asymmetry can be established independently of cilia, notably through the intrinsic chirality of the acto-myosin cytoskeleton. Here, we show that Myosin1D (Myo1D), a previously identified regulator of Drosophila LR asymmetry, is essential for the formation and function of the zebrafish LR organizer (LRO), Kupffer's vesicle (KV). Myo1D controls the orientation of LRO cilia and interacts functionally with the planar cell polarity (PCP) pathway component VanGogh-like2 (Vangl2), to shape a productive LRO flow. Our findings identify Myo1D as an evolutionarily conserved regulator of animal LR asymmetry, and show that functional interactions between Myo1D and PCP are central to the establishment of animal LR asymmetry.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping