PUBLICATION
The inhibition of GSK-3β promotes the production of reactive oxygen species via β-catenin/C/EBPα signaling in the spleen of zebrafish (Danio rerio)
- Authors
- Liu, D., Yu, H., Gao, L., Li, A., Deng, H., Zhang, Z., Tao, S., Liu, Z., Yang, Q., Pang, Q.
- ID
- ZDB-PUB-180227-4
- Date
- 2018
- Source
- Fish & shellfish immunology 76: 110-120 (Journal)
- Registered Authors
- Keywords
- CCAAT/Enhancer binding protein α, Danio rerio, Glycogen synthase kinase-3β, Reactive oxygen species, β-catenin
- MeSH Terms
-
- Signal Transduction*
- Reactive Oxygen Species/metabolism*
- Random Allocation
- Lithium/adverse effects
- Zebrafish/genetics*
- Zebrafish/immunology*
- Spleen/immunology*
- Fish Proteins/antagonists & inhibitors*
- Fish Proteins/genetics
- Fish Proteins/metabolism
- beta Catenin/genetics
- beta Catenin/metabolism
- Glycogen Synthase Kinase 3 beta/antagonists & inhibitors*
- Animals
- CCAAT-Enhancer-Binding Protein-alpha/genetics
- CCAAT-Enhancer-Binding Protein-alpha/metabolism
- PubMed
- 29477497 Full text @ Fish Shellfish Immunol.
Citation
Liu, D., Yu, H., Gao, L., Li, A., Deng, H., Zhang, Z., Tao, S., Liu, Z., Yang, Q., Pang, Q. (2018) The inhibition of GSK-3β promotes the production of reactive oxygen species via β-catenin/C/EBPα signaling in the spleen of zebrafish (Danio rerio). Fish & shellfish immunology. 76:110-120.
Abstract
In this study, the mechanism that the inhibition of glycogen synthase kinase-3β (GSK-3β) promotes the production of reactive oxygen species (ROS) via β-catenin/CCAAT/enhancer binding protein α (C/EBPα) signaling was investigated in the spleen of zebrafish (Danio rerio). The results demonstrated that the inhibition of GSK-3β induced the mRNA expression of β-catenin and C/EBPα by lithium (Li) treatments or GSK-3β RNA interference. The levels of hydrogen peroxide (H2O2), superoxide anion (O2.-), and hydroxy radical (·OH) as well as the activity of superoxide dismutase (SOD) were increased, while the activities of catalase (CAT) and glutathione peroxidase (GSH-PX) were decreased in the spleen and ZF4 cells of zebrafish by Li+ treatments. In addition, GSK-3β RNA interference increased ROS levels and decreased the activities of CAT and GSH-PX in the spleen. The fluorescence intensity of ROS was increased but the mitochondrial membrane potential (MMP) was decreased by Li+ treatments in ZF4 cells labeled with 2',7'-dichlorofluorescein diacetate (DCFH-DA) and Rhodamine-123, respectively. The results of present study indicated that the inhibition of GSK-3β promoted the ROS production via β-catenin/C/EBPα signaling in the spleen of zebrafish, and the balance between ROS and antioxidants could be destroyed by the GSK-3β/β-catenin/C/EBPα signaling. The results may be a valuable contribution to understanding the modulatory mechanism of GSK-3β/β-catenin/C/EBPα signaling on the antioxidant system in fish species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping