PUBLICATION
Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome
- Authors
- Modrell, M.S., Lyne, M., Carr, A.R., Zakon, H.H., Buckley, D., Campbell, A.S., Davis, M.C., Micklem, G., Baker, C.V.
- ID
- ZDB-PUB-170328-4
- Date
- 2017
- Source
- eLIFE 6: (Journal)
- Registered Authors
- Keywords
- developmental biology, neuroscience, stem cells
- MeSH Terms
-
- Animal Structures/embryology*
- Gene Expression Regulation, Developmental*
- Animals
- Vertebrates/embryology*
- Gene Expression Profiling
- Sequence Analysis, RNA
- PubMed
- 28346141 Full text @ Elife
Citation
Modrell, M.S., Lyne, M., Carr, A.R., Zakon, H.H., Buckley, D., Campbell, A.S., Davis, M.C., Micklem, G., Baker, C.V. (2017) Insights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptome. eLIFE. 6.
Abstract
The anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish (Polyodon spathula). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Cav channel and rectifying Kv channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping