PUBLICATION
Effects of Chronic Perfluorooctanoic Acid (PFOA) at Low Concentration on Morphometrics, Gene Expression, and Fecundity in Zebrafish (Danio rerio)
- Authors
- Jantzen, C.E., Toor, F., Annunziato, K.A., Cooper, K.R.
- ID
- ZDB-PUB-170202-5
- Date
- 2017
- Source
- Reproductive toxicology (Elmsford, N.Y.) 69: 34-42 (Journal)
- Registered Authors
- Keywords
- PFOA, Zebrafish Fecundity, Zebrafish Reproduction, Zebrafish development
- MeSH Terms
-
- Animals
- Water Pollutants, Chemical/toxicity*
- Female
- Zebrafish*/genetics
- Zebrafish*/growth & development
- Zebrafish*/physiology
- Fertility/drug effects
- Embryonic Development/drug effects
- Caprylates/toxicity*
- Male
- Gene Expression Regulation, Developmental/drug effects
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Fluorocarbons/toxicity*
- PubMed
- 28143724 Full text @ Reprod. Toxicol.
- CTD
- 28143724
Citation
Jantzen, C.E., Toor, F., Annunziato, K.A., Cooper, K.R. (2017) Effects of Chronic Perfluorooctanoic Acid (PFOA) at Low Concentration on Morphometrics, Gene Expression, and Fecundity in Zebrafish (Danio rerio). Reproductive toxicology (Elmsford, N.Y.). 69:34-42.
Abstract
Perfluorooctanoic acid (PFOA) is a persistent, toxic, anthropogenic chemical recalcitrant to biodegradation. Based on previous studies in lower and higher vertebrates, it was hypothesized that chronic, sub-lethal, embryonic exposure to PFOA in zebrafish (Danio rerio) would adversely impact fish development, survival, and fecundity. Zebrafish embryo/sac-fry were water exposed to 2.0 or 0nM PFOA from 3 to 120hpf, and juvenile to adult cohorts were fed spiked food (8 pM) until 6 months. After chronic exposure, PFOA exposed fish were significantly smaller in total weight and length. Gene expression analysis found a significant decrease of transporters slco2b1, slco4a1, slco3a1 and tgfb1a, and a significant increase of slco1d1 expression. PFOA exposed fish produced significantly fewer eggs with reduced viability and developmental stage delay in F1. Chronic, low-dose exposure of zebrafish to PFOA significantly altered normal development, survival and fecundity and would likely impact wild fish population fitness in watersheds chronically exposed to PFOA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping