PUBLICATION
Wnt signaling balances specification of the cardiac and pharyngeal muscle fields
- Authors
- Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S.
- ID
- ZDB-PUB-170115-6
- Date
- 2017
- Source
- Mechanisms of Development 143: 32-41 (Journal)
- Registered Authors
- Waxman, Joshua
- Keywords
- Cardiac, Mesoderm patterning, Organ fields, Pharyngeal muscle, Wnt signaling, Zebrafish
- MeSH Terms
-
- Actins/genetics
- Actins/metabolism
- Gene Expression Regulation, Developmental*
- Stem Cells/cytology
- Stem Cells/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Transcription Factor 7-Like 1 Protein/genetics
- Transcription Factor 7-Like 1 Protein/metabolism
- Homeobox Protein Nkx-2.5/genetics
- Homeobox Protein Nkx-2.5/metabolism
- Embryo, Nonmammalian
- Cytoskeletal Proteins/genetics*
- Cytoskeletal Proteins/metabolism
- Pharyngeal Muscles/cytology
- Pharyngeal Muscles/embryology
- Pharyngeal Muscles/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Signal Transduction
- Body Patterning/genetics*
- Animals, Genetically Modified
- Animals
- Wnt Proteins/genetics*
- Wnt Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- Mesoderm/cytology
- Mesoderm/embryology
- Mesoderm/metabolism
- Myocardium/cytology
- Myocardium/metabolism*
- PubMed
- 28087459 Full text @ Mech. Dev.
Citation
Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S. (2017) Wnt signaling balances specification of the cardiac and pharyngeal muscle fields. Mechanisms of Development. 143:32-41.
Abstract
Canonical Wnt/β-catenin (Wnt) signaling plays multiple conserved roles during fate specification of cardiac progenitors in developing vertebrate embryos. Although lineage analysis in ascidians and mice has indicated there is a close relationship between the cardiac second heart field (SHF) and pharyngeal muscle (PM) progenitors, the signals underlying directional fate decisions of the cells within the cardio-pharyngeal muscle field in vertebrates are not yet understood. Here, we examined the temporal requirements of Wnt signaling in cardiac and PM development. In contrast to a previous report in chicken embryos that suggested Wnt inhibits PM development during somitogenesis, we find that in zebrafish embryos Wnt signaling is sufficient to repress PM development during anterior-posterior patterning. Importantly, the temporal sensitivity of dorso-anterior PMs to increased Wnt signaling largely overlaps with when Wnt signaling promotes specification of the adjacent cardiac progenitors. Furthermore, we find that excess early Wnt signaling can cell autonomously promote expansion of the first heart field (FHF) progenitors at the expense of PM and SHF within the anterior lateral plate mesoderm (ALPM). Our study provides insight into an antagonistic developmental mechanism that balances the sizes of the adjacent cardiac and PM progenitor fields in early vertebrate embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping