PUBLICATION
Prometryn and humic acid induce Cytochrome P450 1A expression in Danio rerio (zebrafish)
- Authors
- Zhao, Q., Shi, F., Zhu, L.
- ID
- ZDB-PUB-160930-1
- Date
- 2017
- Source
- Ecotoxicology and environmental safety 135: 40-47 (Journal)
- Registered Authors
- Keywords
- CYP 1A, EROD, HA, Herbicides, Tissues, Zebrafish
- MeSH Terms
-
- Animals
- Cytochrome P-450 CYP1A1/genetics*
- Gills/drug effects
- Gills/enzymology
- Humic Substances/toxicity*
- Liver/drug effects
- Liver/enzymology
- Prometryne/toxicity*
- Receptors, Aryl Hydrocarbon/genetics
- Transcription, Genetic/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 27685669 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhao, Q., Shi, F., Zhu, L. (2017) Prometryn and humic acid induce Cytochrome P450 1A expression in Danio rerio (zebrafish). Ecotoxicology and environmental safety. 135:40-47.
Abstract
Humic acid (HA) is a major component of dissolved organic matter, is ubiquitous in the aquatic environment and influences the biological toxicity of organic pollutants. In this study, we investigated the cytochrome P450 1A (CYP 1A) mRNA expression and ethoxyresorufin-O-deethylase (EROD) activity in the gills and liver of zebrafish following exposure to the s-triazine herbicide prometryn with or without HA present. Prometryn induced both CYP 1A mRNA expression and EROD activity. The CYP 1A mRNA expression of zebrafish that were exposed to a combination of prometryn and HA was increased compared to those exposed to prometryn alone. A likely cause for CYP 1A induction is the impact of special components of HA, functioning as aryl hydrocarbon receptor (AHR) agonists. In combination with HA, these increase prometryn levels in tissues. Similar results for EROD activity were evident. In our time course study, CYP 1A mRNA expression reached maximum values during 24h. This revealed CYP 1A mRNA transcription as a comparatively sensitive toxicity index. In a recovery experiment, we found a faster decrease of CYP 1A mRNA expression to control levels (CK) in gills compared to liver tissue. Following exposure to HA, CYP 1A mRNA expression in liver tissue displayed a faster decrease to CK levels. HA induced enhanced metabolic rates for prometryn. In contrast, recovery regularity of CYP 1A expression in gills was independent of the presence of HA. This result indicates different detoxification mechanisms for HA in liver and gills.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping