PUBLICATION
l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model
- Authors
- Szabó, E.R., Plangár, I., Tőkés, T., Mán, I., Polanek, R., Kovács, R., Fekete, G., Szabó, Z., Csenki, Z., Baska, F., Hideghéty, K.
- ID
- ZDB-PUB-160804-3
- Date
- 2016
- Source
- Zebrafish 13(6): 481-488 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Radiation
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/radiation effects
- Glycerylphosphorylcholine/pharmacology*
- Interleukin-1beta/metabolism
- Lethal Dose 50
- Models, Animal
- NF-kappa B/metabolism
- Radiation, Ionizing*
- Radiation-Protective Agents/pharmacology*
- Real-Time Polymerase Chain Reaction
- Zebrafish/physiology*
- PubMed
- 27486826 Full text @ Zebrafish
Citation
Szabó, E.R., Plangár, I., Tőkés, T., Mán, I., Polanek, R., Kovács, R., Fekete, G., Szabó, Z., Csenki, Z., Baska, F., Hideghéty, K. (2016) l-Alpha Glycerylphosphorylcholine as a Potential Radioprotective Agent in Zebrafish Embryo Model. Zebrafish. 13(6):481-488.
Abstract
This work establishes the zebrafish embryo model for ionizing radiation (IR) modifier research and also evaluates the protective effect of l-alpha glycerylphosphorylcholine (GPC). Embryos were exposed to a single-fraction whole-body gamma irradiation (5, 10, 15, and 20 Gy) at different postfertilization time points and were serially assessed for viability and macro- and micromorphologic abnormalities. After toxicity evaluation, 194 μM of GPC was added for certain groups with 3-h incubation before the radiation. Nuclear factor kappa B (NF-κB) and interleukin-1β (IL-1β) expression changes were measured using quantitative real-time polymerase chain reaction. A higher sensitivity could be observed at earlier stages of the embryogenesis. The lethal dose (LD50) for 6 hours postfertilization (hpf) embryos was 15 Gy and for 24 hpf was 20 Gy on day 7, respectively. GPC administration resulted in a significant improvement in both the distortion rate and survival of the 24 hpf embryos. Qualitative evaluation of the histological changes confirmed the protective effect of GPC. IL-1β and NF-κB overexpression due to 10 Gy irradiation was also reduced by GPC. GPC exhibited promising radioprotective effects in our zebrafish embryo model, decreasing the irradiation-induced morphological damage and lethality with significant reduction of IR-caused pro-inflammatory activation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping