PUBLICATION
Knockdown of zebrafish Nanog increases proliferation of primordial germ cells during early embryonic development
- Authors
- Wang, H., Liu, Y., Ye, D., Li, J., Liu, J., Deng, F.
- ID
- ZDB-PUB-160430-11
- Date
- 2016
- Source
- Development, growth & differentiation 58(4): 355-66 (Journal)
- Registered Authors
- Ye, Ding
- Keywords
- PGCs, embryonic development, proliferation, zNanog, zebrafish
- MeSH Terms
-
- Animals
- Germ Cells/metabolism*
- Gene Knockdown Techniques
- Zebrafish/embryology*
- Embryonic Development/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Nanog Homeobox Protein/genetics
- Nanog Homeobox Protein/metabolism*
- PubMed
- 27125179 Full text @ Dev. Growth Diff.
Citation
Wang, H., Liu, Y., Ye, D., Li, J., Liu, J., Deng, F. (2016) Knockdown of zebrafish Nanog increases proliferation of primordial germ cells during early embryonic development. Development, growth & differentiation. 58(4):355-66.
Abstract
Nanog is a homeodomain transcription factor that plays a prominent role in maintaining the pluripotency and self-renewal capacity of embryonic stem cells (ESCs) in mammals. Medaka Nanog is necessary for S-phase transition and proliferation during embryonic development. However, whether Nanog regulates the proliferation of primordial germ cells (PGCs) during embryonic development has not yet been investigated. In this study, we identified the homologue of the mammalian Nanog gene in zebrafish (zNanog). The expression of both zNanog mRNA and protein was demonstrated in the spermatogonia (male germ stem cells) of the testis and the early oocytes of the ovary. During the embryonic development, zNanog mRNA is expressed in the cytoplasm of PGCs, and its protein is localized to the PGC nuclei. We also found that zNanog depletion using morpholinos resulted in the increases and aberrant localization of PGCs in the zebrafish embryos from the sphere stage to the 50% epiboly stage. These data indicated that zNanog inhibits the PGCs proliferation in early embryonic development of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping