PUBLICATION
Poly(A)-binding proteins are required for microRNA-mediated silencing and to promote target deadenylation in C. elegans
- Authors
- Flamand, M.N., Wu, E., Vashisht, A., Jannot, G., Keiper, B.D., Simard, M.J., Wohlschlegel, J., Duchaine, T.F.
- ID
- ZDB-PUB-160421-10
- Date
- 2016
- Source
- Nucleic acids research 44(12): 5924-35 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- Adenosine Monophosphate/metabolism
- Animals
- Binding Sites
- Caenorhabditis elegans/genetics*
- Caenorhabditis elegans/growth & development
- Caenorhabditis elegans/metabolism
- Caenorhabditis elegans Proteins/genetics*
- Caenorhabditis elegans Proteins/metabolism
- Embryo, Nonmammalian
- Gene Silencing
- Larva/genetics*
- Larva/growth & development
- Larva/metabolism
- MicroRNAs/genetics*
- MicroRNAs/metabolism
- Poly A/genetics*
- Poly A/metabolism
- Poly(A)-Binding Protein I/genetics*
- Poly(A)-Binding Protein I/metabolism
- Poly(A)-Binding Protein II/genetics*
- Poly(A)-Binding Protein II/metabolism
- Protein Binding
- Protein Biosynthesis
- RNA-Induced Silencing Complex/genetics
- RNA-Induced Silencing Complex/metabolism
- PubMed
- 27095199 Full text @ Nucleic Acids Res.
Citation
Flamand, M.N., Wu, E., Vashisht, A., Jannot, G., Keiper, B.D., Simard, M.J., Wohlschlegel, J., Duchaine, T.F. (2016) Poly(A)-binding proteins are required for microRNA-mediated silencing and to promote target deadenylation in C. elegans. Nucleic acids research. 44(12):5924-35.
Abstract
Cytoplasmic poly(A)-binding proteins (PABPs) link mRNA 3' termini to translation initiation factors, but they also play key roles in mRNA regulation and decay. Reports from mice, zebrafish andDrosophilafurther involved PABPs in microRNA (miRNA)-mediated silencing, but through seemingly distinct mechanisms. Here, we implicate the twoCaenorhabditis elegansPABPs (PAB-1 and PAB-2) in miRNA-mediated silencing, and elucidate their mechanisms of action using concerted genetics, protein interaction analyses, and cell-free assays. We find thatC. elegansPABPs are required for miRNA-mediated silencing in embryonic and larval developmental stages, where they act through a multi-faceted mechanism. Depletion of PAB-1 and PAB-2 results in loss of both poly(A)-dependent and -independent translational silencing. PABPs accelerate miRNA-mediated deadenylation, but this contribution can be modulated by 3'UTR sequences. While greater distances with the poly(A) tail exacerbate dependency on PABP for deadenylation, more potent miRNA-binding sites partially suppress this effect. Our results refine the roles of PABPs in miRNA-mediated silencing and support a model wherein they enable miRNA-binding sites by looping the 3'UTR poly(A) tail to the bound miRISC and deadenylase.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping