PUBLICATION
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
- Authors
- Fu, Q., Martin, B.L., Matus, D.Q., Gao, L.
- ID
- ZDB-PUB-160324-7
- Date
- 2016
- Source
- Nature communications 7: 11088 (Journal)
- Registered Authors
- Martin, Benjamin
- Keywords
- Biological sciences, Biotechnology, Cell biology
- MeSH Terms
-
- Animals
- Caenorhabditis elegans*
- Embryo, Nonmammalian/anatomy & histology*
- Imaging, Three-Dimensional/methods*
- Microscopy/methods*
- Zebrafish*
- PubMed
- 27004937 Full text @ Nat. Commun.
Citation
Fu, Q., Martin, B.L., Matus, D.Q., Gao, L. (2016) Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy. Nature communications. 7:11088.
Abstract
Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping