PUBLICATION
Open-top selective plane illumination microscope for conventionally mounted specimens
- Authors
- McGorty, R., Liu, H., Kamiyama, D., Dong, Z., Guo, S., Huang, B.
- ID
- ZDB-PUB-150721-1
- Date
- 2015
- Source
- Optics express 23: 16142-16153 (Journal)
- Registered Authors
- Dong, Zhiqiang, Guo, Su
- Keywords
- none
- MeSH Terms
-
- Lighting/instrumentation*
- Equipment Failure Analysis
- Specimen Handling/instrumentation*
- Refractometry/instrumentation*
- Equipment Design
- Sensitivity and Specificity
- Imaging, Three-Dimensional/instrumentation
- Image Enhancement/instrumentation*
- Intravital Microscopy/instrumentation*
- Reproducibility of Results
- PubMed
- 26193587 Full text @ Opt. Express
Citation
McGorty, R., Liu, H., Kamiyama, D., Dong, Z., Guo, S., Huang, B. (2015) Open-top selective plane illumination microscope for conventionally mounted specimens. Optics express. 23:16142-16153.
Abstract
We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.
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