PUBLICATION
Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging
- Authors
- Berlin, S., Carroll, E.C., Newman, Z.L., Okada, H.O., Quinn, C.M., Kallman, B., Rockwell, N.C., Martin, S.S., Lagarias, J.C., Isacoff, E.Y.
- ID
- ZDB-PUB-150715-11
- Date
- 2015
- Source
- Nature Methods 12(9): 852-8 (Journal)
- Registered Authors
- Isacoff, Ehud
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Calcium Signaling/physiology*
- Cell Tracking/methods
- Cells, Cultured
- Drosophila
- Light
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism*
- Microscopy, Fluorescence/methods
- Neurons/cytology*
- Neurons/physiology*
- Optogenetics/methods*
- Protein Engineering/methods
- Rats
- Zebrafish
- PubMed
- 26167640 Full text @ Nat. Methods
Citation
Berlin, S., Carroll, E.C., Newman, Z.L., Okada, H.O., Quinn, C.M., Kallman, B., Rockwell, N.C., Martin, S.S., Lagarias, J.C., Isacoff, E.Y. (2015) Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Nature Methods. 12(9):852-8.
Abstract
Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demonstrated in cultured neurons and in fruit fly and zebrafish larvae how single cells could be selected out of dense populations for visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is transferrable to other sensors based on circularly permutated GFP (cpGFP).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping