PUBLICATION

Development and origins of Zebrafish ocular vasculature

Authors
Kaufman, R., Weiss, O., Sebbagh, M., Ravid, R., Gibbs-Bar, L., Yaniv, K., Inbal, A.
ID
ZDB-PUB-150419-5
Date
2015
Source
BMC Developmental Biology   15: 18 (Journal)
Registered Authors
Inbal, Adi, Yaniv, Karina
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/embryology*
  • Eye/blood supply*
  • Zebrafish/physiology*
PubMed
25888280 Full text @ BMC Dev. Biol.
Abstract
The developing eye receives blood supply from two vascular systems, the intraocular hyaloid system and the superficial choroidal vessels. In zebrafish, a highly stereotypic and simple set of vessels develops on the surface of the eye prior to development of choroidal vessels. The origins and formation of this so-called superficial system have not been described.
We have analyzed the development of superficial vessels by time-lapse imaging and identified their origins by photoconversion experiments in kdrl:Kaede transgenic embryos. We show that the entire superficial system is derived from a venous origin, and surprisingly, we find that the hyaloid system has, in addition to its previously described arterial origin, a venous origin for specific vessels. Despite arising solely from a vein, one of the vessels in the superficial system, the nasal radial vessel (NRV), appears to acquire an arterial identity while growing over the nasal aspect of the eye and this happens in a blood flow-independent manner.
Our results provide a thorough analysis of the early development and origins of zebrafish ocular vessels and establish the superficial vasculature as a model for studying vascular patterning in the context of the developing eye.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping