PUBLICATION
Evidence of common cadmium and copper uptake routes in zebrafish Danio rerio
- Authors
- Komjarova, I., Bury, N.R.
- ID
- ZDB-PUB-141008-4
- Date
- 2014
- Source
- Environmental science & technology 48(21): 12946-51 (Journal)
- Registered Authors
- Bury, Nicolas
- Keywords
- none
- MeSH Terms
-
- Adenosine Triphosphatases/genetics
- Adenosine Triphosphatases/metabolism
- Animals
- Cadmium/pharmacokinetics*
- Cation Transport Proteins/genetics
- Cation Transport Proteins/metabolism*
- Copper/pharmacokinetics*
- Gene Expression Regulation/drug effects
- Gills/drug effects*
- Gills/physiology
- Membrane Transport Proteins/genetics
- Membrane Transport Proteins/metabolism
- Sodium-Hydrogen Exchangers/genetics
- Sodium-Hydrogen Exchangers/metabolism
- TRPV Cation Channels/genetics
- TRPV Cation Channels/metabolism
- Water Pollutants, Chemical/pharmacokinetics*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 25289693 Full text @ Env. Sci. Tech.
Citation
Komjarova, I., Bury, N.R. (2014) Evidence of common cadmium and copper uptake routes in zebrafish Danio rerio. Environmental science & technology. 48(21):12946-51.
Abstract
Cadmium and copper accumulations in gills of zebrafish were measured during a 48 h exposure to 0.025 µM 106Cd and 0.05 or 0.5 µM 65Cu as a single metal or their mixtures. The gill transcript levels of genes involved in the transport of Cu (CTR1 and ATP7A), Na (NHE-2), Ca (ECaC), divalent metals (DMT1) and Zn (ZIP8) were also compared between treatments at 24 and 48 h. Cd uptake was significantly suppressed in the presence of Cu, indicating interaction between Cu and Cd at uptake sites, but Cu uptake was unaffected by Cd. The decrease in Cd accumulation rates in the presence of Cu was associated with an increase in transcript abundance of ECaC at 24h and DMT1 at 48h, and a decrease in Zip8 transcript levels, all known as routes for Cd uptake. Fish exposed to 0.5 µM 65Cu show an increase in gill ATP7a transcript abundance, suggesting that Cu is removed from the gill and is transferred to other organs for detoxification. A reduction in gill CTR1 transcript abundance was observed during the Cu-Cd exposure; this may be a regulatory mechanism to reduce Cu loading if Cu is entering the gills by other uptake routes, such as ECaC and DMT1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping