PUBLICATION
Multiplexed aberration measurement for deep tissue imaging in vivo
- Authors
- Wang, C., Liu, R., Milkie, D.E., Sun, W., Tan, Z., Kerlin, A., Chen, T.W., Kim, D.S., Ji, N.
- ID
- ZDB-PUB-170214-301
- Date
- 2014
- Source
- Nature Methods 11: 1037-40 (Journal)
- Registered Authors
- Keywords
- Ca2+ imaging, Fluorescence imaging, Multiphoton microscopy, Optical imaging
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Caenorhabditis elegans
- Fluorescent Dyes/chemistry
- Fourier Analysis
- Histones/chemistry
- Light*
- Mice
- Mice, Inbred C57BL
- Microscopy, Fluorescence/methods
- Neuroimaging/methods*
- Optics and Photonics*
- Protein Processing, Post-Translational
- Proteins/chemistry
- Pupil/physiology
- Visual Cortex/physiology
- Zebrafish
- PubMed
- 25128976 Full text @ Nat. Methods
Citation
Wang, C., Liu, R., Milkie, D.E., Sun, W., Tan, Z., Kerlin, A., Chen, T.W., Kim, D.S., Ji, N. (2014) Multiplexed aberration measurement for deep tissue imaging in vivo. Nature Methods. 11:1037-40.
Abstract
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping