PUBLICATION
Effects of Lactobacillus Rhamnosus GG on Hepatic and Serum Lipid Profiles in Zebrafish Exposed to Ethanol
- Authors
- Schneider, A.C., Machado, A.B., de Assis, A.M., Hermes, D.M., Schaefer, P.G., Guizzo, R., Fracasso, L.B., de-Paris, F., Meurer, F., Barth, A.L., da Silveira, T.R.
- ID
- ZDB-PUB-140706-22
- Date
- 2014
- Source
- Zebrafish 11(4): 371-8 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Ethanol/toxicity*
- Female
- Intestines/microbiology
- Lacticaseibacillus rhamnosus/metabolism*
- Lipid Metabolism/drug effects
- Lipids/blood
- Liver/drug effects
- Male
- Probiotics/metabolism*
- Zebrafish/blood
- Zebrafish/metabolism
- Zebrafish/microbiology*
- PubMed
- 24987799 Full text @ Zebrafish
Citation
Schneider, A.C., Machado, A.B., de Assis, A.M., Hermes, D.M., Schaefer, P.G., Guizzo, R., Fracasso, L.B., de-Paris, F., Meurer, F., Barth, A.L., da Silveira, T.R. (2014) Effects of Lactobacillus Rhamnosus GG on Hepatic and Serum Lipid Profiles in Zebrafish Exposed to Ethanol. Zebrafish. 11(4):371-8.
Abstract
Abstract Zebrafish is a powerful tool in pharmacological research and useful to identify new therapies. Probiotics can offer therapeutic options in alcoholic liver disease. This study was done in two independent experiments: first, we confirmed the intestinal colonization of probiotic Lactobacillus rhamnosus GG (LGG) after ethanol exposure. Second, four groups were performed: control (C), probiotic (P), ethanol (E), and probiotic+ethanol (P+E). Liver histology, hepatocytes morphometry, hepatic and serum lipid quantifications were conducted in second experiment. During 4 weeks, P and P+E groups were fed with LGG supplemented feed; E and C unsupplemented. E and P+E groups received 0.5% of ethanol added into tank water. Zebrafish exposed to ethanol (E group) presented intense liver steatosis after 28 days in contrast to the almost normalized liver histology of P+E group at the same period. Liver morphometry showed a significant enlargement of hepatocytes of E group after 4 weeks (p<0.0001). Serum triglycerides decreased in P+E group compared with C, P (p<0.001), and E (p=0.004), after 14 and 28 days similarly. Serum cholesterol was also decreased by LGG; P group decreased compared with C and E after 14 days (p=0.002 and p=0.007, respectively) and P+E group decreased significantly compared with E and C groups (p<0.0001) after 28 days. Hepatic triglycerides were reduced in P+E group after 28 days compared to E (p=0.006). The persistence of LGG in zebrafish intestines was demonstrated. LGG decreased serum levels of triglycerides and cholesterol and improved hepatic steatosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping