PUBLICATION
Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
- Authors
- Prevedel, R., Yoon, Y.G., Hoffmann, M., Pak, N., Wetzstein, G., Kato, S., Schrödel, T., Raskar, R., Zimmer, M., Boyden, E.S., Vaziri, A.
- ID
- ZDB-PUB-140520-8
- Date
- 2014
- Source
- Nature Methods 11(7): 727-30 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Caenorhabditis elegans
- Calcium/metabolism*
- Calcium Signaling
- Imaging, Three-Dimensional/methods*
- Larva/ultrastructure
- Microscopy/methods*
- Microscopy, Fluorescence/methods
- Neurons/physiology*
- Zebrafish
- PubMed
- 24836920 Full text @ Nat. Methods
Citation
Prevedel, R., Yoon, Y.G., Hoffmann, M., Pak, N., Wetzstein, G., Kato, S., Schrödel, T., Raskar, R., Zimmer, M., Boyden, E.S., Vaziri, A. (2014) Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy. Nature Methods. 11(7):727-30.
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm x 700 μm x 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping