PUBLICATION
Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos
- Authors
- Griepenburg, J.C., Ruble, B.K., and Dmochowski, I.J.
- ID
- ZDB-PUB-130708-37
- Date
- 2013
- Source
- Bioorganic & Medicinal Chemistry 21(20): 6198-6204 (Journal)
- Registered Authors
- Keywords
- microRNA (miRNA), Let-7, zebrafish, antagomir
- MeSH Terms
-
- Zebrafish
- Embryo, Nonmammalian
- MicroRNAs/chemistry*
- MicroRNAs/genetics
- Oligonucleotides/chemistry*
- Oligonucleotides/genetics
- Animals
- PubMed
- 23721917 Full text @ Bioorg. Med. Chem.
Citation
Griepenburg, J.C., Ruble, B.K., and Dmochowski, I.J. (2013) Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos. Bioorganic & Medicinal Chemistry. 21(20):6198-6204.
Abstract
Many biological functions of microRNA (miRNA) have been identified in the past decade. However, a single miRNA can regulate multiple gene targets, thus it has been a challenge to elucidate the specific functions of each miRNA in different locations and times. New chemical tools make it possible to modulate miRNA activity with higher spatiotemporal resolution. Here, we describe light-activated (caged) constructs for switching let-7 miRNA ‘on’ or ‘off’ with 365 nm light in developing zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping