PUBLICATION

Light sheet microscopy for real-time developmental biology

Authors
Weber, M., and Huisken, J.
ID
ZDB-PUB-111019-7
Date
2011
Source
Current opinion in genetics & development   21(5): 566-72 (Journal)
Registered Authors
Huisken, Jan
Keywords
none
MeSH Terms
  • Animals
  • Developmental Biology/instrumentation*
  • Developmental Biology/methods*
  • Microscopy/instrumentation*
  • Microscopy/methods*
  • Time Factors
PubMed
21963791 Full text @ Curr. Opin. Genet. Dev.
Abstract
Within only a few short years, light sheet microscopy has contributed substantially to the emerging field of real-time developmental biology. Low photo-toxicity and high-speed multiview acquisition have made selective plane illumination microscopy (SPIM) a popular choice for studies of organ morphogenesis and function in zebrafish, Drosophila, and other model organisms. A multitude of different light sheet microscopes have emerged for the noninvasive imaging of specimens ranging from single molecules to cells, tissues, and entire embryos. In particular, developmental biology can benefit from the ability to watch developmental events occur in real time in an entire embryo, thereby advancing our understanding on how cells form tissues and organs. However, it presents a new challenge to our existing data and image processing tools. This review gives an overview of where we stand as light sheet microscopy branches out, explores new areas, and becomes more specialized.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping