PUBLICATION
Ecotoxicogenomics to support ecological risk assessment: A case study with bisphenol A in fish
- Authors
- Villeneuve, D.L., Garcia-Reyero, N., Escalon, B.L., Jensen, K.M., Cavallin, J.E., Makynen, E., Durhan, E., Kahl, M.D., Thomas, L.M., Perkins, E.J., and Ankley, G.T.
- ID
- ZDB-PUB-110803-8
- Date
- 2012
- Source
- Environmental science & technology 46(1): 51-59 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE28351, GEO:GSE28354
- MeSH Terms
-
- Animals
- Benzhydryl Compounds
- Cyprinidae/blood
- Cyprinidae/genetics*
- Ecotoxicology/methods*
- Female
- Gene Expression Regulation/drug effects
- Male
- Metagenomics/methods*
- Oligonucleotide Array Sequence Analysis
- Phenols/toxicity*
- Principal Component Analysis
- Reproducibility of Results
- Risk Assessment
- Toxicity Tests*
- Transcriptome/drug effects
- Transcriptome/genetics
- Vitellogenins/blood
- Zebrafish/genetics*
- PubMed
- 21786754 Full text @ Env. Sci. Tech.
- CTD
- 21786754
Citation
Villeneuve, D.L., Garcia-Reyero, N., Escalon, B.L., Jensen, K.M., Cavallin, J.E., Makynen, E., Durhan, E., Kahl, M.D., Thomas, L.M., Perkins, E.J., and Ankley, G.T. (2012) Ecotoxicogenomics to support ecological risk assessment: A case study with bisphenol A in fish. Environmental science & technology. 46(1):51-59.
Abstract
Effects of bisphenol A (BPA) on ovarian transcript profiles as well as targeted endpoints with endocrine/reproductive relevance were examined in two fish species, fathead minnow (Pimephales promelas) and zebrafish (Danio rerio), exposed in parallel using matched experimental designs. Four days of waterborne exposure to 10 µg BPA caused significant vitellogenin induction in both species. However, zebrafish were less sensitive to effects on hepatic gene expression and steroid production than fathead minnow and the magnitude of vitellogenin induction was more modest (i.e., 3-fold compared to 13000-fold in fathead minnow). The concentration-response at the ovarian transcriptome level was non-monotonic and violated assumptions that underlie proposed methods for estimating hazard thresholds from transcriptomic results. However, the non-monotonic profile was consistent among species and there were nominal similarities in the functions associated with the differentially expressed genes, suggesting potential activation of common pathway perturbation motifs in both species. Overall, the results provide an effective case study for considering the potential application of ecotoxicogenomics to ecological risk assessments and provide novel comparative data regarding effects of BPA in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping