PUBLICATION
Deep and fast live imaging with two-photon scanned light-sheet microscopy
- Authors
- Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M., and Fraser, S.E.
- ID
- ZDB-PUB-110719-41
- Date
- 2011
- Source
- Nature Methods 8(9): 757-60 (Journal)
- Registered Authors
- Fraser, Scott E., Koos, David
- Keywords
- none
- MeSH Terms
-
- Animals
- Drosophila melanogaster
- Embryo, Nonmammalian/ultrastructure*
- Imaging, Three-Dimensional/instrumentation
- Imaging, Three-Dimensional/methods*
- Lasers
- Light/adverse effects
- Microscopy/methods*
- Zebrafish
- PubMed
- 21765409 Full text @ Nat. Methods
Citation
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M., and Fraser, S.E. (2011) Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods. 8(9):757-60.
Abstract
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping