PUBLICATION
Isolation and characterization of major histocompatibility class IIβ genes in an endangered North American cyprinid fish, the Rio Grande silvery minnow (Hybognathus amarus)
- Authors
- Osborne, M.J., and Turner, T.F.
- ID
- ZDB-PUB-110503-12
- Date
- 2011
- Source
- Fish & shellfish immunology 30(6): 1275-1282 (Journal)
- Registered Authors
- Keywords
- MHC class IIβ, Rio Grande silvery minnow, Cyprinidae
- MeSH Terms
-
- Genes, MHC Class II/genetics*
- Base Sequence
- Models, Genetic
- DNA Primers/genetics
- Amino Acid Sequence
- Animals
- Phylogeny*
- Alleles
- Cyprinidae/genetics*
- Likelihood Functions
- Genetic Variation*
- Evolution, Molecular*
- Molecular Sequence Data
- DNA, Complementary/genetics
- Sequence Analysis, DNA
- Endangered Species*
- PubMed
- 21463692 Full text @ Fish Shellfish Immunol.
Citation
Osborne, M.J., and Turner, T.F. (2011) Isolation and characterization of major histocompatibility class IIβ genes in an endangered North American cyprinid fish, the Rio Grande silvery minnow (Hybognathus amarus). Fish & shellfish immunology. 30(6):1275-1282.
Abstract
The major histocompatibility complex (MHC) is a critical component of the adaptive immune response in vertebrates. Due to the role that MHC plays in immunity, absence of variation within these genes may cause species to be vulnerable to emerging diseases. The freshwater fish family Cyprinidae comprises the most diverse and species-rich group of freshwater fish in the world, but some are imperiled. Despite considerable species richness and the long evolutionary history of the family, there are very few reports of MHC sequences (apart from a few model species), and no sequences are reported from endemic North American cyprinids (subfamily Leuciscinae). Here we isolate and characterize the MH Class II beta genes from complementary DNA and genomic DNA of the non-model, endangered Rio Grande silvery minnow (Hybognathus amarus), a North American cyprinid. Phylogenetic reconstruction revealed two groups of divergent MH alleles that are paralogous to previously described loci found in deeply divergent cyprinid taxa including common carp, zebrafish, African large barb and bream. Both groups of alleles were under the influence of diversifying selection yet not all individuals had alleles belonging to both allelic groups. We concluded that the general organization and pattern of variation of MH class II genes in Rio Grande silvery minnow is similar to that identified in other cyprinid fishes studied to date, despite distant evolutionary relationships and evidence of a severe genetic bottleneck.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping