PUBLICATION
Conserved motif of CDK5RAP2 mediates its localization to centrosomes and the Golgi complex
- Authors
- Wang, Z., Wu, T., Shi, L., Zhang, L., Zheng, W., Qu, J.Y., Niu, R., and Qi, R.Z.
- ID
- ZDB-PUB-100518-15
- Date
- 2010
- Source
- The Journal of biological chemistry 285(29): 22658-22665 (Journal)
- Registered Authors
- Keywords
- Calmodulin, Centrosome, Golgi, Molecular dynamics, Trafficking, CDK5RAP2
- MeSH Terms
-
- Amino Acid Motifs
- Calmodulin/metabolism
- Cell Line, Tumor
- Centrosome/metabolism*
- Conserved Sequence
- Golgi Apparatus/metabolism*
- Humans
- Intracellular Signaling Peptides and Proteins/chemistry*
- Intracellular Signaling Peptides and Proteins/metabolism*
- Mutant Proteins/metabolism
- Nerve Tissue Proteins/chemistry*
- Nerve Tissue Proteins/metabolism*
- Protein Binding
- Protein Transport
- Structure-Activity Relationship
- PubMed
- 20466722 Full text @ J. Biol. Chem.
Citation
Wang, Z., Wu, T., Shi, L., Zhang, L., Zheng, W., Qu, J.Y., Niu, R., and Qi, R.Z. (2010) Conserved motif of CDK5RAP2 mediates its localization to centrosomes and the Golgi complex. The Journal of biological chemistry. 285(29):22658-22665.
Abstract
As the primary microtubule-organizing centers, centrosomes require gamma-tubulin for microtubule nucleation and organization. Located in close vicinity to centrosomes, the Golgi complex is another microtubule-organizing organelle in interphase cells. CDK5RAP2 is a gamma-tubulin complex-binding protein and functions in gamma-tubulin attachment to centrosomes. In this study, we find that CDK5RAP2 localizes to the Golgi complex in an ATP and centrosome-dependent manner and associates with Golgi membranes independent of microtubules. CDK5RAP2 contains a centrosome-targeting domain with its core region highly homologous to the Motif 2 (CM2) of centrosomin, a functionally related protein in Drosophila. However, the sequence of this CDK5RAP2 domain is distinct from other known centrosome-targeting sequences. This sequence, referred to as the CM2-like motif, is also conserved in related proteins in chicken and zebrafish. Therefore, CDK5RAP2 may undertake a conserved mechanism for centrosomal localization. Using mutational approaches, we demonstrate that the CM2-like motif plays a crucial role in the centrosomal and Golgi localization of CDK5RAP2. Although this CDK5RAP2 sequence possesses the activity of Ca2+-independent calmodulin (CaM) binding, CaM binding to this sequence is dispensable for centrosomal and Golgi association. Altogether, CDK5RAP2 may represent a novel mechanism for centrosomal and Golgi localization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping