PUBLICATION

Automated image-based phenotypic analysis in zebrafish embryos

Authors
Vogt, A., Cholewinski, A., Shen, X., Nelson, S.G., Lazo, J.S., Tsang, M., and Hukriede, N.A.
ID
ZDB-PUB-090302-12
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(3): 656-663 (Journal)
Registered Authors
Hukriede, Neil
Keywords
cognition network technology, high-content screening, angiogenesis, pironetin, zebrafish
MeSH Terms
  • Angiogenesis Inhibitors/pharmacology
  • Animals
  • Embryo, Nonmammalian/blood supply
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/embryology*
  • Embryo, Nonmammalian/metabolism*
  • Genes, Reporter/genetics
  • Image Processing, Computer-Assisted/methods*
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
19235725 Full text @ Dev. Dyn.
Abstract
Presently, the zebrafish is the only vertebrate model compatible with contemporary paradigms of drug discovery. Zebrafish embryos are amenable to automation necessary for high-throughput chemical screens, and optical transparency makes them potentially suited for image-based screening. However, the lack of tools for automated analysis of complex images presents an obstacle to using the zebrafish as a high-throughput screening model. We have developed an automated system for imaging and analyzing zebrafish embryos in multi-well plates regardless of embryo orientation and without user intervention. Images of fluorescent embryos were acquired on a high-content reader and analyzed using an artificial intelligence-based image analysis method termed Cognition Network Technology (CNT). CNT reliably detected transgenic fluorescent embryos (Tg(fli1:EGFP)(y1)) arrayed in 96-well plates and quantified intersegmental blood vessel development in embryos treated with small molecule inhibitors of anigiogenesis. The results demonstrate it is feasible to adapt image-based high-content screening methodology to measure complex whole organism phenotypes. Developmental Dynamics 238:656-663, 2009. (c) 2009 Wiley-Liss, Inc.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping