PUBLICATION
Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer
- Authors
- Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L.
- ID
- ZDB-PUB-090311-1
- Date
- 2009
- Source
- Nature Chemical Biology 5(2): 100-107 (Journal)
- Registered Authors
- Amatruda, James F.
- Keywords
- none
- MeSH Terms
-
- Axin Protein
- Humans
- Molecular Structure
- Neoplasms/metabolism*
- Regeneration*
- Repressor Proteins/metabolism
- Signal Transduction/drug effects*
- Signal Transduction/physiology
- Wnt Proteins/metabolism
- Wnt Proteins/physiology*
- beta Catenin/metabolism
- beta Catenin/physiology
- PubMed
- 19125156 Full text @ Nat. Chem. Biol.
Citation
Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology. 5(2):100-107.
Abstract
The pervasive influence of secreted Wnt signaling proteins in tissue homeostasis and tumorigenesis has galvanized efforts to identify small molecules that target Wnt-mediated cellular responses. By screening a diverse synthetic chemical library, we have discovered two new classes of small molecules that disrupt Wnt pathway responses; whereas one class inhibits the activity of Porcupine, a membrane-bound acyltransferase that is essential to the production of Wnt proteins, the other abrogates destruction of Axin proteins, which are suppressors of Wnt/beta-catenin pathway activity. With these small molecules, we establish a chemical genetic approach for studying Wnt pathway responses and stem cell function in adult tissue. We achieve transient, reversible suppression of Wnt/beta-catenin pathway response in vivo, and we establish a mechanism-based approach to target cancerous cell growth. The signal transduction mechanisms shown here to be chemically tractable additionally contribute to Wnt-independent signal transduction pathways and thus could be broadly exploited for chemical genetics and therapeutic goals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping