PUBLICATION
A BBSome Subunit Links Ciliogenesis, Microtubule Stability, and Acetylation
- Authors
- Loktev, A.V., Zhang, Q., Beck, J.S., Searby, C.C., Scheetz, T.E., Bazan, J.F., Slusarski, D.C., Sheffield, V.C., Jackson, P.K., and Nachury, M.V.
- ID
- ZDB-PUB-081218-23
- Date
- 2008
- Source
- Developmental Cell 15(6): 854-865 (Journal)
- Registered Authors
- Slusarski, Diane C.
- Keywords
- CELLBIO
- MeSH Terms
-
- Cytoplasm/metabolism
- Molecular Sequence Data
- Gene Expression Regulation, Developmental*
- Bardet-Biedl Syndrome/metabolism*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/physiology*
- Amino Acid Sequence
- Histone Deacetylases/metabolism
- Cell Line
- Gene Expression Regulation*
- Zebrafish
- Microtubules/metabolism*
- Sequence Homology, Amino Acid
- Acetylation
- Animals
- Cilia/metabolism*
- Carrier Proteins/chemistry*
- Carrier Proteins/physiology*
- Models, Biological
- PubMed
- 19081074 Full text @ Dev. Cell
Citation
Loktev, A.V., Zhang, Q., Beck, J.S., Searby, C.C., Scheetz, T.E., Bazan, J.F., Slusarski, D.C., Sheffield, V.C., Jackson, P.K., and Nachury, M.V. (2008) A BBSome Subunit Links Ciliogenesis, Microtubule Stability, and Acetylation. Developmental Cell. 15(6):854-865.
Abstract
Primary cilium dysfunction affects the development and homeostasis of many organs in Bardet-Biedl syndrome (BBS). We recently showed that seven highly conserved BBS proteins form a stable complex, the BBSome, that functions in membrane trafficking to and inside the primary cilium. We have now discovered a BBSome subunit that we named BBIP10. Similar to other BBSome subunits, BBIP10 localizes to the primary cilium, BBIP10 is present exclusively in ciliated organisms, and depletion of BBIP10 yields characteristic BBS phenotypes in zebrafish. Unexpectedly, BBIP10 is required for cytoplasmic microtubule polymerization and acetylation, two functions not shared with any other BBSome subunits. Strikingly, inhibition of the tubulin deacetylase HDAC6 restores microtubule acetylation in BBIP10-depleted cells, and BBIP10 physically interacts with HDAC6. BBSome-bound BBIP10 may therefore function to couple acetylation of axonemal microtubules and ciliary membrane growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping