PUBLICATION
Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
- Authors
- Huisken, J., and Stainier, D.Y.
- ID
- ZDB-PUB-070907-30
- Date
- 2007
- Source
- Optics letters 32(17): 2608-2610 (Journal)
- Registered Authors
- Stainier, Didier
- Keywords
- none
- MeSH Terms
-
- Absorption
- Animals
- Embryo, Nonmammalian/pathology
- Equipment Design
- Image Processing, Computer-Assisted
- Light
- Microscopy/methods*
- Microscopy, Fluorescence/methods*
- Optics and Photonics
- Scattering, Radiation
- Software
- Time Factors
- Zebrafish
- PubMed
- 17767321 Full text @ Opt. Lett.
Citation
Huisken, J., and Stainier, D.Y. (2007) Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Optics letters. 32(17):2608-2610.
Abstract
Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the sample is compensated by illuminating the sample consecutively from opposing directions. The resulting two images are computationally fused yielding a superior image. The effective light sheet is thinner, and the axial resolution is increased by square root 2 over single-directional SPIM. The multidirectional illumination proves essential in biological specimens such as millimeter-sized embryos. The performance of mSPIM is demonstrated by the imaging of live zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping