PUBLICATION
Mannose 6-phosphate receptors in an ancient vertebrate, zebrafish
- Authors
- Nolan, C.M., McCarthy, K., Eivers, E., Jirtle, R.L., and Byrnes, L.
- ID
- ZDB-PUB-060124-15
- Date
- 2006
- Source
- Development genes and evolution 216(3): 144-151 (Journal)
- Registered Authors
- Byrnes, Lucy, Eivers, Edward, McCarthy, Karena
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Binding Sites
- Carbohydrate Metabolism
- Cations/metabolism
- Cattle
- Chickens/genetics
- Cloning, Molecular
- Conserved Sequence
- Cytoplasmic Structures/metabolism
- Embryo, Nonmammalian
- Embryonic Development
- Gene Expression
- Humans
- Insulin-Like Growth Factor II/metabolism
- Mice
- Molecular Sequence Data
- Neurons/metabolism
- Opossums/genetics
- Phylogeny
- Platypus/genetics
- Receptor, IGF Type 2/genetics*
- Sequence Homology, Amino Acid
- Signal Transduction
- Tissue Distribution
- Zebrafish/genetics*
- PubMed
- 16411117 Full text @ Dev. Genes Evol.
Citation
Nolan, C.M., McCarthy, K., Eivers, E., Jirtle, R.L., and Byrnes, L. (2006) Mannose 6-phosphate receptors in an ancient vertebrate, zebrafish. Development genes and evolution. 216(3):144-151.
Abstract
The endosome/lysosome system plays key roles in embryonic development, but difficulties posed by inaccessible mammalian embryos have hampered detailed studies. The accessible, transparent embryos of Danio rerio, together with the genetic and experimental approaches possible with this organism, provide many advantages over rodents. In mammals, mannose 6-phosphate receptors (MPRs) target acid hydrolases to endosomes and lysosomes, but nothing is known of acid hydrolase targeting in zebrafish. Here, we describe the sequence of the zebrafish cation-dependent MPR (CD-MPR) and cation-independent MPR (CI-MPR), and compare them with their mammalian orthologs. We show that all residues critical for mannose 6-phosphate (M6P) recognition are present in the extracellular domains of the zebrafish receptors, and that trafficking signals in the cytoplasmic tails are also conserved. This suggests that the teleost receptors possess M6P binding sites with properties similar to those of mammalian MPRs, and that targeting of lysosomal enzymes by MPRs represents an ancient pathway in vertebrate cell biology. We also determined the expression patterns of the CD-MPR and CI-MPR during embryonic development in zebrafish. Both genes are expressed from the one-cell stage through to the hatching period. In early embryos, expression is ubiquitous, but in later stages, expression of both receptors is restricted to the anterior region of the embryo, covering the forebrain, midbrain and hindbrain. The expression patterns suggest time- and tissue-specific functions for the receptors, with particular evidence for roles in neural development. Our study establishes zebrafish as a novel, genetically tractable model for in vivo studies of MPR function and lysosome biogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping