PUBLICATION

Asymmetry of intronic pre-miRNA structures in functional RISC assembly

Authors
Lin, S.L., Chang, D., and Ying, S.Y.
ID
ZDB-PUB-050714-5
Date
2005
Source
Gene   356: 32-38 (Journal)
Registered Authors
Chang, Donald C.
Keywords
microRNA (miRNA); RNA interference (RNAi); RNA-induced gene silencing complex (RISC); intron; zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Genetic Vectors/genetics
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Introns/genetics*
  • MicroRNAs/chemistry
  • MicroRNAs/genetics*
  • MicroRNAs/metabolism
  • Nucleic Acid Conformation
  • RNA Interference
  • RNA Precursors/chemistry
  • RNA Precursors/genetics*
  • RNA Splicing
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • RNA-Induced Silencing Complex/chemistry
  • RNA-Induced Silencing Complex/genetics*
  • Transfection
  • Zebrafish
PubMed
16005165 Full text @ Gene
Abstract
The two oligonucleotide strands of a siRNA duplex are functionally asymmetric in assembling the RNAi effector, RNA-induced gene silencing complex (RISC). Based on this asymmetric RISC assembly model in vitro, formation of a microRNA (miRNA) and complementary miRNA (miRNA*) duplex was proposed to be an essential step for the assembly of miRNA-associated RISC (miRISC). We observed here that a strong structural bias exists in the selection of a mature miRNA strand for RISC assembly in zebrafish using an intronic miRNA-like vector to target EGFP mRNA for regulation. The position of the stemloop in a precursor miRNA (pre-miRNA) was involved in the determination of miRNA-miRNA* asymmetry of the pre-miRNA stemarm, leading to different miRNA maturation during miRISC assembly. These findings suggest that the miRISC assembly is likely different from the RISC assembly model of siRNA in zebrafish, providing the first in vivo evidence for asymmetric miRISC assembly.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping