PUBLICATION
The lamin CxxM motif promotes nuclear membrane growth
- Authors
- Prüfert, K., Vogel, A., and Krohne, G.
- ID
- ZDB-PUB-041122-4
- Date
- 2004
- Source
- Journal of Cell Science 117(Pt.25): 6105-6116 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Transfection
- Green Fluorescent Proteins/metabolism
- Lamin Type A/chemistry*
- Lamin Type A/genetics*
- Xenopus
- Cell Membrane/metabolism
- Humans
- Mutation
- Nuclear Envelope/metabolism*
- Animals
- Cysteine/chemistry
- Drosophila/metabolism
- Amino Acid Motifs
- Zebrafish
- Cell Proliferation
- Electrophoresis, Polyacrylamide Gel
- Transcription, Genetic
- Drosophila melanogaster/genetics
- Microscopy, Electron
- Microscopy, Fluorescence
- Protein Structure, Tertiary
- Cell Nucleus/metabolism*
- Lamin Type B/chemistry
- Lamin Type B/genetics*
- COS Cells
- PubMed
- 15546914 Full text @ J. Cell Sci.
Citation
Prüfert, K., Vogel, A., and Krohne, G. (2004) The lamin CxxM motif promotes nuclear membrane growth. Journal of Cell Science. 117(Pt.25):6105-6116.
Abstract
We analyzed the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins. Lamins containing a CxxM motif in their primary sequence (lamins A, B2, Dm0) induced the formation of lobulated nuclei with multi-membrane-layered, highly folded nuclear membranes and intranuclear membrane assemblies, as observed by electron microscopy. Such morphological alterations were not observed with Drosophila lamin C, a lamin without this motif or with a lamin B2 mutant (B2-SxxM) where the cysteine of the CxxM motif is replaced by a serine. Drosophila lamin C mutants containing a CxxM motif behaved like B-type lamins thus confirming that this tetrapeptide is directly involved in the morphological changes we observed. Nuclear membrane proliferation could also be induced by lamin B2 in COS-7 cells and in zebrafish embryos but not by human lamin A in COS-7 cells. We speculate that the human lamin A is incompletely processed in Xenopus A6 cells and therefore behaves in this cell line like a B-type lamin. Our results indicate that the CxxM motif of B-type lamins has a dual function: it mediates lamin targeting to the inner nuclear membrane thereby promoting nuclear membrane growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping