PUBLICATION
accordion, a zebrafish behavioral mutant, has a muscle relaxation defect due to a mutation in the ATPase Ca2+ pump SERCA1
- Authors
- Hirata, H., Saint-Amant, L., Waterbury, J., Cui, W., Zhou, W., Li, Q., Goldman, D., Granato, M., and Kuwada, J.Y.
- ID
- ZDB-PUB-041008-10
- Date
- 2004
- Source
- Development (Cambridge, England) 131(21): 5457-5468 (Journal)
- Registered Authors
- Cui, Wilson, Goldman, Dan, Granato, Michael, Hirata, Hiromi, Kuwada, John, Li, Qin, Saint-Amant, Louis, Waterbury, Julie, Zhou, Weibin
- Keywords
- Zebrafish, Accordion, Behavior, Muscle, Calcium, SERCA1, Brody disease
- MeSH Terms
-
- Muscles/physiology*
- Calcium-Transporting ATPases/chemistry
- Calcium-Transporting ATPases/genetics*
- Calcium-Transporting ATPases/metabolism*
- Gene Expression Regulation, Developmental
- Humans
- Morphogenesis
- Time Factors
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/physiology*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/physiology
- Behavior, Animal/physiology*
- Electrophysiology
- Animals
- Sarcoplasmic Reticulum Calcium-Transporting ATPases
- Amino Acid Sequence
- Mutation/genetics*
- Neuromuscular Junction/metabolism
- Sequence Alignment
- Molecular Sequence Data
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Calcium/metabolism
- Muscle Relaxation/physiology
- Central Nervous System/metabolism
- PubMed
- 15469975 Full text @ Development
Citation
Hirata, H., Saint-Amant, L., Waterbury, J., Cui, W., Zhou, W., Li, Q., Goldman, D., Granato, M., and Kuwada, J.Y. (2004) accordion, a zebrafish behavioral mutant, has a muscle relaxation defect due to a mutation in the ATPase Ca2+ pump SERCA1. Development (Cambridge, England). 131(21):5457-5468.
Abstract
When wild-type zebrafish embryos are touched at 24 hours post-fertilization (hpf), they typically perform two rapid alternating coils of the tail. By contrast, accordion (acc) mutants fail to coil their tails normally but contract the bilateral trunk muscles simultaneously to shorten the trunk, resulting in a pronounced dorsal bend. Electrophysiological recordings from muscles showed that the output from the central nervous system is normal in mutants, suggesting a defect in muscles is responsible. In fact, relaxation in acc muscle is significantly slower than normal. In vivo imaging of muscle Ca(2+) transients revealed that cytosolic Ca(2+) decay was significantly slower in acc muscle. Thus, it appears that the mutant behavior is caused by a muscle relaxation defect due to the impairment of Ca(2+) re-uptake. Indeed, acc mutants carry a mutation in atp2a1 gene that encodes the sarco(endo)plasmic reticulum Ca(2+)-ATPase 1 (SERCA1), a Ca(2+) pump found in the muscle sarcoplasmic reticulum (SR) that is responsible for pumping Ca(2+) from the cytosol back to the SR. As SERCA1 mutations in humans lead to Brody disease, an exercise-induced muscle relaxation disorder, zebrafish accordion mutants could be a useful animal model for this condition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping