PUBLICATION
Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development
- Authors
- Scherberich, A., Tucker, R.P., Samandari, E., Brown-Luedi, M., Martin, D., and Chiquet-Ehrismann, R.
- ID
- ZDB-PUB-040109-8
- Date
- 2004
- Source
- Journal of Cell Science 117(4): 1-581 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Recombinant Proteins/genetics
- Recombinant Proteins/isolation & purification
- Recombinant Proteins/metabolism
- DNA, Complementary/genetics
- DNA, Complementary/isolation & purification
- Kidney/embryology
- Kidney/metabolism*
- Muscle, Smooth/embryology
- Muscle, Smooth/metabolism*
- Phylogeny
- Cell Adhesion/physiology
- Organ Specificity
- Amino Acid Sequence
- Humans
- Mice
- Bone and Bones/embryology
- Bone and Bones/metabolism*
- Cloning, Molecular
- Immunohistochemistry
- Molecular Sequence Data
- Zebrafish
- Tenascin/analogs & derivatives
- Tenascin/biosynthesis
- Tenascin/genetics*
- Tenascin/isolation & purification
- Zebrafish Proteins/genetics
- Cells, Cultured
- In Situ Hybridization
- Gene Expression Regulation, Developmental*
- PubMed
- 14709716 Full text @ J. Cell Sci.
Citation
Scherberich, A., Tucker, R.P., Samandari, E., Brown-Luedi, M., Martin, D., and Chiquet-Ehrismann, R. (2004) Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development. Journal of Cell Science. 117(4):1-581.
Abstract
We cloned and characterized a novel member of the tenascin family of extracellular matrix proteins - the murine orthologue of zebrafish tenascin-W. Full-length recombinant tenascin-W was expressed and purified from mammalian cell cultures. Rotary shadowing followed by electron microscopy showed that tenascin-W forms hexabrachions. We studied its expression during development and in the adult by immunohistochemistry, in situ hybridization and immunoblotting. Tenascin-W is expressed during palate formation, osteogenesis and smooth muscle development. In the adult, tenascin-W is found in the kidney, cardiac semilunar valves, corneal limbus and periosteum. Tenascin-W and tenascin-C expression overlap in many of these areas. Bone-morphogenic-protein-2 treated C2C12 cells secrete tenascin-W and are able to adhere to and to extend actin-rich processes on a tenascin-W substratum. In vitro, cells bind to tenascin-W in an RGD-dependent manner. This adhesion is increased by transfection of alpha8 integrin, which localizes with tenascin-W in the periosteum and kidney.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping