PUBLICATION
Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development
- Authors
- Jones, I., Rogers, S.A., Kille, P., and Sweeney, G.E.
- ID
- ZDB-PUB-020403-22
- Date
- 2002
- Source
- General and comparative endocrinology 125(2): 226-235 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cloning, Molecular*
- Salmo salar/embryology
- Salmo salar/growth & development*
- Salmo salar/metabolism
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics*
- Reverse Transcriptase Polymerase Chain Reaction
- Gene Expression*
- Amino Acid Sequence
- RNA, Messenger/analysis
- Molecular Sequence Data
- Oncorhynchus mykiss/embryology
- Oncorhynchus mykiss/growth & development*
- Oncorhynchus mykiss/metabolism
- Receptors, Thyroid Hormone*
- Sequence Alignment
- Base Sequence
- Receptors, Cytoplasmic and Nuclear/chemistry
- Receptors, Cytoplasmic and Nuclear/genetics*
- Larva/growth & development
- Larva/metabolism
- PubMed
- 11884068 Full text @ Gen. Comp. Endocrinol.
Citation
Jones, I., Rogers, S.A., Kille, P., and Sweeney, G.E. (2002) Molecular cloning and expression of thyroid hormone receptor alpha during salmonid development. General and comparative endocrinology. 125(2):226-235.
Abstract
Thyroid hormones have been implicated as important regulators of teleost development. To gain a better understanding of the potential roles of the thyroid system in salmonids a genomic clone which encoded rainbow trout TR-alpha was isolated. This clone exhibited highest amino acid identity to Japanese flounder TR-alphaB (94%) and zebrafish TR-alpha1 (94%). Oligonucleotides were designed against the rainbow trout sequence and the complete coding region of Atlantic salmon TR-alpha was isolated by RACE-PCR. The Atlantic salmon sequence exhibited highest amino acid identity to rainbow trout TR-alpha (98%), Japanese flounder TR-alphaB (93%), and zebrafish TR-alpha1 (90%). Atlantic salmon TR-alpha exhibited the classic modular structure associated with members of the nuclear receptor superfamily and consisted of a divergent A/B domain while the DNA and ligand-binding domains were highly conserved to other teleost TR proteins. Temporal expression from the rainbow trout TR-alpha gene was monitored by semiquantitative RT-PCR at selected stages during rainbow trout embryonic and larval development. High levels of maternal transcripts were present at cleavage (Stage 6) which were rapidly degraded by gastrulation (Stage 13). Low levels of TR-alpha expression were then detected during organogenesis (Stages 20, 24, 26, 29, and 31). A peak in mRNA levels was observed at hatch (Stage 32) after which levels rose in a gradual manner during larval development (Stages 33, 34, 35, and 36) to reach maximal values at first feeding (Stage 37). These results suggest that the thyroid axis is functional and that embryonic and larval rainbow trout are at least capable of responding to thyroid hormones. These observations implicate the thyroid system as being an important regulator of salmonid development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping