PUBLICATION
Two isoforms of vasa homologs in a teleost fish: their differential expression during germ cell differentiation
- Authors
- Kobayashi, T., Kajiura-Kobayashi, H., and Nagahama, Y.
- ID
- ZDB-PUB-020306-2
- Date
- 2002
- Source
- Mechanisms of Development 111(1-2): 167-171 (Journal)
- Registered Authors
- Keywords
- vasa; isoform; primordial germ cell; germ cell; gonadal differentiation; sex differentiation; oogenesis; spermatogenesis; mRNA; protein subcellular localization; immunohistochemistry; teleost; tilapia; zebrafish; trout
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Differentiation/genetics
- Embryo, Nonmammalian
- Female
- Gene Expression Regulation, Developmental
- Male
- Molecular Sequence Data
- Oocytes/cytology
- Oocytes/physiology*
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA Helicases/genetics*
- RNA Helicases/metabolism*
- Sequence Homology, Amino Acid
- Spermatozoa/cytology
- Spermatozoa/physiology*
- Tilapia/embryology
- Tilapia/genetics*
- PubMed
- 11804791 Full text @ Mech. Dev.
Citation
Kobayashi, T., Kajiura-Kobayashi, H., and Nagahama, Y. (2002) Two isoforms of vasa homologs in a teleost fish: their differential expression during germ cell differentiation. Mechanisms of Development. 111(1-2):167-171.
Abstract
Two isoforms of vasa mRNA and protein are present in a teleost fish, tilapia. One (vas-s) lacks a part of the N-terminal region found in the other isoform (vas). Both isoforms are expressed in oocytes through the embryonic stage when primordial germ cells (PGCs) localize in the lateral plate mesoderm. After PGC localization in the gonadal anlagen, vas-s expression increased and vas expression became undetectable. Expression of both isoforms was observed again after morphological gonadal sex differentiation, irrespective of genotypic sex. In ovary, compared with vas expression vas-s expression predominated throughout oogenesis. In testis, vas expression was predominant compared with vas-s during spermatogenesis. These results indicate that relative expression of two vasa isoforms is dependent upon germ cell differentiation and sex.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping