PUBLICATION
An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system
- Authors
- Matsumoto, M., Saito, T., Takasaki, J., Kamohara, M., Sugimoto, T., Kobayashi, M., Tadokoro, M., Matsumoto, S.-I., Ohishi, T., and Furuichi, K.
- ID
- ZDB-PUB-000623-13
- Date
- 2000
- Source
- Biochemical and Biophysical Research Communications 272(2): 576-582 (Journal)
- Registered Authors
- Keywords
- G-protein coupled receptor; evolution; central nervous system; genital organs; amine-like receptor; zebrafish; human chromosome locus; SREB; subfamily
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Biogenic Amines/metabolism
- Brain/metabolism*
- CHO Cells
- Chromosome Mapping
- Chromosomes, Human/genetics
- Cloning, Molecular
- Conserved Sequence/genetics*
- Cricetinae
- Expressed Sequence Tags
- Gene Expression Profiling
- Heterotrimeric GTP-Binding Proteins/metabolism*
- Humans
- Introns/genetics
- Ligands
- Lod Score
- Molecular Sequence Data
- Multigene Family/genetics*
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism*
- Open Reading Frames/genetics
- RNA, Messenger/analysis
- RNA, Messenger/genetics
- Rats
- Receptors, Cell Surface/genetics*
- Receptors, Cell Surface/metabolism*
- Sequence Alignment
- Zebrafish/genetics
- PubMed
- 10833454 Full text @ Biochem. Biophys. Res. Commun.
Citation
Matsumoto, M., Saito, T., Takasaki, J., Kamohara, M., Sugimoto, T., Kobayashi, M., Tadokoro, M., Matsumoto, S.-I., Ohishi, T., and Furuichi, K. (2000) An evolutionarily conserved G-protein coupled receptor family, SREB, expressed in the central nervous system. Biochemical and Biophysical Research Communications. 272(2):576-582.
Abstract
We report here a novel family of G-protein coupled receptor (GPCR) which is extraordinarily conserved among vertebrate species. This family, designated SREB (Super Conserved Receptor Expressed in Brain), consists of at least three members, termed SREB1, SREB2, and SREB3. SREB members share 52-63% amino acid identity with each other and show relatively high similarity to previously known amine amine GPCRs (approximately 25% identity). Amino acid sequence identity between human and rat orthologues is 97% for SREB1 and 99% for SREB3, while the SREB2 sequence is surprisingly completely identical between the species. Furthermore, amino acid sequence of zebrafish SREB2 and SREB3 are 94 and 78% identical to mammal orthologues. Northern blot analysis revealed that SREB members are predominantly expressed in the brain regions and genital organs. Radiation hybrid analysis localized SREB1, SREB2, and SREB3 genes to different human chromosomes, namely 3p21-p14, 7q31 and Xp11, respectively. The high sequence conservation and abundant expression in the central nervous system suggest the existence of undiscovered fundamental neuronal systems consisting of SREB family members and their endogenous ligand(s).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping