PUBLICATION
Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain
- Authors
- Mechta-Grigoriou, F., Garel, S., and Charnay, P.
- ID
- ZDB-PUB-101027-49
- Date
- 2000
- Source
- Development (Cambridge, England) 127(1): 119-128 (Journal)
- Registered Authors
- Charnay, Patrick
- Keywords
- none
- MeSH Terms
-
- 3T3 Cells
- Animals
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/physiology
- Early Growth Response Protein 2
- Feedback
- Gene Expression Regulation, Developmental*
- Mice
- Neoplasm Proteins*
- Repressor Proteins/genetics*
- Rhombencephalon/embryology*
- Rhombencephalon/pathology
- Transcription Factors/genetics*
- Transcription Factors/physiology
- Zebrafish
- PubMed
- 10654606 Full text @ Development
Citation
Mechta-Grigoriou, F., Garel, S., and Charnay, P. (2000) Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain. Development (Cambridge, England). 127(1):119-128.
Abstract
The developing vertebrate hindbrain is transiently subdivided along the anterior-posterior axis into metameric units, called rhombomeres (r). These segments constitute units of lineage restriction and display specific gene expression patterns. The transcription factor gene Krox-20 is restricted to r3 and r5, and is required for the development of these rhombomeres. We present evidence that Krox-20 transcriptional activity is under the control of a negative feedback mechanism in the hindbrain. This regulatory loop involves two closely related proteins, Nabl and Nab2, previously identified as antagonists of Krox-20 transcriptional activity in cultured cells. Here we show that in the mouse hindbrain, Nab1 and Nab2 recapitulate the Krox-20 expression pattern and that their expression is dependent on Krox-20 function. Furthermore, misexpression of Nab1 or Nab2 in zebrafish embryos leads to alterations in the expression patterns of several hindbrain markers, consistent with an inhibition of Krox-20 activity. Taken together, these data indicate that Krox-20 positively regulates the expression of its own antagonists and raise the possibility that this negative feedback regulatory loop may play a role in the control of hindbrain development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping