FIGURE SUMMARY
Title

Zebrafish as a platform to evaluate the potential of lipidic nanoemulsions for gene therapy in cancer

Authors
Cascallar, M., Hurtado, P., Lores, S., Pensado-López, A., Quelle-Regaldie, A., Sánchez, L., Piñeiro, R., de la Fuente, M.
Source
Full text @ Front Pharmacol
FIGURE 1

1% agarose gel electrophoresis (80 V, 40 min) to evaluate the association efficacy between PSN and nucleic acids: miR (2 µg) and pDNA (0.2 µg).

FIGURE 2

RT-qPCR results of the effect of miR 145 associated and non-associated with PSN in the relative expression of sox9b and gata6 genes in zebrafish embryos.

FIGURE 3

Confocal images of zebrafish embryos after a 72 h incubation with TopFluor-PSN (in green) associated and non-associated with Cy5-labelled miR and pDNA (in blue).

FIGURE 4

Confocal images of in vivo biodistribution of TopFluor-labelled nanoemulsions (green) with and without miR-Cy5 and pDNA (blue), after 48 h incubation in microinjected 48 hpf zebrafish embryos.

FIGURE 5

Images of the in vivo interaction between nanoemulsions, labelled with TopFluor (green), and associated and non-associated miR and pDNA Cy5-labelled (blue) with DiI-MDA-MB-231 cancer cells (red), by confocal microscopy.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Pharmacol
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