Train error rate (A) and test error rate (B) with p-cutoff evaluation results with 500,000 iterations in zebrafish heart chambers. The statistical difference was analyzed using ordinary one-way ANOVA, followed by Dunn’s multiple comparison test. The different letters (a and b) indicate significant differences with p < 0.05.

The representative image plots of the ventricle chamber labeled by DLC. (A) Eight different circle points label the ventricle of zebrafish larvae. (B) Plot created from normal placement without interference. (C) Plot created from a microscope slide that was moving slightly. (D) Plot created from the zebrafish heart that suddenly moved.

Cardiac physiology parameter comparison between DLC, ImageJ TSA, and Kymograph methods in zebrafish. Results for heart rate (A), end-diastolic volume (B), end-systolic volume (C), normalized stroke volume (D), cardiac output (E), shortening fraction (F), and ejection fraction (G), from three methods were compared statistically by RM one-way ANOVA followed by Tukey’s multiple comparisons test. Data are expressed as mean ± SD and significant differences (p < 0.05) are indicated by lower case a, b, and c (n = 15).

Cardiac physiology parameter comparison between control, ethanol 2%, and ponatinib 2.5 ppm analyzed by DLC method. Heart rate (A), end-diastolic volume (B), end-systolic volume (C), normalized stroke volume (D), cardiac output (E), shortening fraction (F), and ejection fraction (G). Results from control and chemical treatment were statistically analyzed by ordinary one-way ANOVA followed by Fisher’s LSD test as post hoc multiple comparison test. Data are expressed as mean ± SD and statistical difference is indicated by * p < 0.05; *** p < 0.001; and **** p < 0.0001 (n = 20).

Heart rate variability evaluation in zebrafish embryos after exposure to ethanol 2% and ponatinib 2.5 ppm. Data analyses were conducted based on the standard deviation 1 (sd1) (A) and standard deviation 2 (sd2) (B) of the heart chamber generated by Poincaré Plot (C–E). The sd1 and sd2 results were statistically analyzed by Kruskal–Wallis test, followed by the uncorrected Dunn’s test. Data are expressed as mean ± SD, and statistical difference is indicated by **** p < 0.0001 (n = 20).