Indications of transgenic phenotype in a photographic image of male zebrafish, changes in body weight, and sperm kinetic parameters of gh-transgenic F0104 strain zebrafish (Danio rerio) and non-transgenic strain. (A)gh-transgenic zebrafish male from F0104 strain showing the ubiquitous and constitutive distribution of GFP expression; non-transgenic zebrafish shown in white arrows; (B) bodyweight characteristics; (C) total motile sperm; (D) sperm motility period. All p-values < 0.05 are summarized with one asterisk and < 0.0001 with three asterisks. Results are statistically expressed by mean ± standard error of the mean (SEM) (n = 10 for each experimental group). NT, non-transgenic; GH+, gh-transgenic; WL, water line; BB, bottom of beaker.